PDF(Pubmed)
Abstract：
UNASSIGNED: Acute respiratory distress syndrome (ARDS) is caused by severe pulmonary inflammation and the leading cause of death in the intensive care unit.
UNASSIGNED: We used single-cell RNA sequencing to compare peripheral blood mononuclear cells from sepsis-induced ARDS (SEP-ARDS) and pneumonic ARDS (PNE-ARDS) patient. Then, we used the GSE152978 and GSE152979 datasets to identify molecular dysregulation mechanisms at the transcriptional level in ARDS.
UNASSIGNED: Markedly increased CD14 cells were the predominant immune cell type observed in SEP-ARDS and PNE-ARDS patients. Cytotoxic cells and natural killer (NK) T cells were exclusively identified in patients with PNE-ARDS. An enrichment analysis of differentially expressed genes (DEGs) suggested that Th1 cell differentiation and Th2 cell differentiation were enriched in cytotoxic cells, and that the IL-17 signaling pathway, NOD receptor signaling pathway, and complement and coagulation cascades were enriched in CD14 cells. Furthermore, according to GSE152978 and GSE152979, 1939 DEGs were identified in patients with ARDS and controls; they were mainly enriched in the Kyoto Encyclopedia of Genes and Genomes pathways. RBP7 had the highest area under the curve values among the 12 hub genes and was mainly expressed in CD14 cells. Additionally, hub genes were negatively correlated with NK cells and positively correlated with neutrophils, cytotoxic cells, B cells, and macrophages.
UNASSIGNED: A severe imbalance in the proportion of immune cells and immune dysfunction were observed in SEP-ARDS and PNE-ARDS patients. RBP7 may be immunologically associated with CD14 cells and serve as a potential marker of ARDS.
UNASSIGNED: We used single-cell RNA sequencing to compare peripheral blood mononuclear cells from sepsis-induced ARDS (SEP-ARDS) and pneumonic ARDS (PNE-ARDS) patient. Then, we used the GSE152978 and GSE152979 datasets to identify molecular dysregulation mechanisms at the transcriptional level in ARDS.
UNASSIGNED: Markedly increased CD14 cells were the predominant immune cell type observed in SEP-ARDS and PNE-ARDS patients. Cytotoxic cells and natural killer (NK) T cells were exclusively identified in patients with PNE-ARDS. An enrichment analysis of differentially expressed genes (DEGs) suggested that Th1 cell differentiation and Th2 cell differentiation were enriched in cytotoxic cells, and that the IL-17 signaling pathway, NOD receptor signaling pathway, and complement and coagulation cascades were enriched in CD14 cells. Furthermore, according to GSE152978 and GSE152979, 1939 DEGs were identified in patients with ARDS and controls; they were mainly enriched in the Kyoto Encyclopedia of Genes and Genomes pathways. RBP7 had the highest area under the curve values among the 12 hub genes and was mainly expressed in CD14 cells. Additionally, hub genes were negatively correlated with NK cells and positively correlated with neutrophils, cytotoxic cells, B cells, and macrophages.
UNASSIGNED: A severe imbalance in the proportion of immune cells and immune dysfunction were observed in SEP-ARDS and PNE-ARDS patients. RBP7 may be immunologically associated with CD14 cells and serve as a potential marker of ARDS.
摘要：
■急性呼吸窘迫综合征（ARDS）是由严重的肺部炎症引起的，是重症监护病房的主要死亡原因。
■我们使用单细胞RNA测序来比较脓毒症诱导的ARDS（SEP-ARDS）和肺炎性ARDS（PNE-ARDS）患者的外周血单核细胞。然后，我们使用GSE152978和GSE152979数据集来鉴定ARDS转录水平的分子失调机制.
■显著增加的CD14细胞是在SEP-ARDS和PNE-ARDS患者中观察到的主要免疫细胞类型。细胞毒性细胞和自然杀伤（NK）T细胞在PNE-ARDS患者中被专门鉴定。对差异表达基因（DEGs）的富集分析表明，Th1细胞分化和Th2细胞分化在细胞毒性细胞中富集，IL-17信号通路,NOD受体信号通路,补体和凝血级联富集在CD14细胞中。此外，根据GSE152978和GSE152979,1939年在ARDS患者和对照组中发现了DEGs;它们主要富集在《京都基因和基因组百科全书》中。RBP7在12个hub基因中具有最高的曲线下面积值,并且主要在CD14细胞中表达。此外,hub基因与NK细胞呈负相关，与中性粒细胞呈正相关，细胞毒性细胞，B细胞,和巨噬细胞。
■在SEP-ARDS和PNE-ARDS患者中观察到免疫细胞比例严重失衡和免疫功能障碍。RBP7可能与CD14细胞免疫相关，并作为ARDS的潜在标志物。
■我们使用单细胞RNA测序来比较脓毒症诱导的ARDS（SEP-ARDS）和肺炎性ARDS（PNE-ARDS）患者的外周血单核细胞。然后，我们使用GSE152978和GSE152979数据集来鉴定ARDS转录水平的分子失调机制.
■显著增加的CD14细胞是在SEP-ARDS和PNE-ARDS患者中观察到的主要免疫细胞类型。细胞毒性细胞和自然杀伤（NK）T细胞在PNE-ARDS患者中被专门鉴定。对差异表达基因（DEGs）的富集分析表明，Th1细胞分化和Th2细胞分化在细胞毒性细胞中富集，IL-17信号通路,NOD受体信号通路,补体和凝血级联富集在CD14细胞中。此外，根据GSE152978和GSE152979,1939年在ARDS患者和对照组中发现了DEGs;它们主要富集在《京都基因和基因组百科全书》中。RBP7在12个hub基因中具有最高的曲线下面积值,并且主要在CD14细胞中表达。此外,hub基因与NK细胞呈负相关，与中性粒细胞呈正相关，细胞毒性细胞，B细胞,和巨噬细胞。
■在SEP-ARDS和PNE-ARDS患者中观察到免疫细胞比例严重失衡和免疫功能障碍。RBP7可能与CD14细胞免疫相关，并作为ARDS的潜在标志物。
