Abstract：
The process of testis development in mammals is accompanied by the proliferation and maturation of Sertoli, Leydig and germ cells. Spermatogenesis depends on hormone regulation, which must bind to a receptor to exert its biological effects. The changes in Hu sheep testis cell composition and FSHR, LHR and AR expression during different developmental stages are unclear (newborn, puberty and adulthood). To address this, using single-cell RNA sequencing, we analyzed testis cell composition and hormone receptor expression changes during three important developmental stages of Hu sheep. We observed significant changes in the composition of somatic and germ cells in different Hu sheep testis developmental stages. Furthermore, we analyzed the FSHR, LHR and AR distribution and expression changes at three important periods and verified them by qRT-PCR and immunofluorescence. Our results suggest that after birth, the proportion of germ cells increased gradually, peaking in adulthood; the proportion of Sertoli cells decreased gradually, reaching the lowest in adulthood; and the proportion of Leydig cells increased and then decreased, reaching the lowest in adulthood. In addition, FSHR, LHR and AR are mainly located in Sertoli, Leydig and germ cells. LHR and FSHR expression decreased with increasing age, while AR expression increased and then decreased with increasing age.
摘要：
哺乳动物的睾丸发育过程伴随着Sertoli的增殖和成熟，Leydig和生殖细胞。精子发生取决于激素调节，必须与受体结合才能发挥其生物学作用。湖羊睾丸细胞组成和FSHR的变化,不同发育阶段的LHR和AR表达尚不清楚(新生，青春期和成年期)。为了解决这个问题，使用单细胞RNA测序，我们分析了湖羊三个重要发育阶段的睾丸细胞组成和激素受体表达的变化。我们观察到不同湖羊睾丸发育阶段体细胞和生殖细胞组成的显著变化。此外，我们分析了FSHR,LHR和AR在三个重要时期的分布和表达变化，并通过qRT-PCR和免疫荧光验证。我们的结果表明，出生后，生殖细胞的比例逐渐增加，成年期达到高峰；支持细胞的比例逐渐降低，在成年后达到最低；睾丸间质细胞的比例先上升后下降，在成年后达到最低。此外,FSHR,LHR和AR主要位于Sertoli，Leydig和生殖细胞。LHR和FSHR表达随年龄增长而降低,AR的表达随着年龄的增长而增加，然后下降。
