PDF(Pubmed)
Abstract：
In resource-limited settings, patients are often first presented to clinical settings when seriously ill and access to proper clinical microbial diagnostics is often very limited or non-existing. On February 16th, 2022 we were on a field trip to test a completely field-deployable metagenomics sequencing set-up, that includes DNA purification, sequencing, and bioinformatics analyses using bioinformatics tools installed on a laptop for water samples, just outside Moshi, Tanzania. On our way to the test site, we were contacted by the nearby Machame hospital regarding a child seriously ill with diarrhea and not responding to treatment. Within the same day, we conducted an onsite metagenomics examination of a fecal sample from the child, and Campylobacter jejuni was identified as the causative agent. The treatment was subsequently changed, with almost immediate improvement, and the child was discharged on February 21st.
摘要：
在资源有限的设置中，患者通常在重病时首先被送往临床环境,获得适当的临床微生物诊断的机会通常非常有限或根本不存在.2月16日,2022年，我们进行了实地考察，以测试完全可现场部署的宏基因组学测序设置，包括DNA纯化,测序,测序使用安装在笔记本电脑上的生物信息学工具对水样进行生物信息学分析，就在莫希外面，坦桑尼亚。在我们去试验场的路上,附近的Machame医院联系了我们，询问一名患有严重腹泻且对治疗无反应的儿童。同一天内,我们对孩子的粪便样本进行了现场宏基因组学检查，空肠弯曲杆菌被确定为病原体。随后改变了治疗方法，随着几乎立即的改善，孩子于2月21日出院。
