PDF(Pubmed)
Abstract：
UNASSIGNED: Flavonoids, as secondary metabolites in plants, play important roles in many biological processes and responses to environmental factors.
UNASSIGNED: Apricot fruits are rich in flavonoid compounds, and in this study, we performed a combined metabolomic and transcriptomic analysis of orange flesh (JN) and white flesh (ZS) apricot fruits.
UNASSIGNED: A total of 222 differentially accumulated flavonoids (DAFs) and 15855 differentially expressed genes (DEGs) involved in flavonoid biosynthesis were identified. The biosynthesis of flavonoids in apricot fruit may be regulated by 17 enzyme-encoding genes, namely PAL (2), 4CL (9), C4H (1), HCT (15), C3\'H (4), CHS (2), CHI (3), F3H (1), F3\'H (CYP75B1) (2), F3\'5\'H (4), DFR (4), LAR (1), FLS (3), ANS (9), ANR (2), UGT79B1 (6) and CYP81E (2). A structural gene-transcription factor (TF) correlation analysis yielded 3 TFs (2 bHLH, 1 MYB) highly correlated with 2 structural genes. In addition, we obtained 26 candidate genes involved in the biosynthesis of 8 differentially accumulated flavonoids metabolites in ZS by weighted gene coexpression network analysis. The candidate genes and transcription factors identified in this study will provide a highly valuable molecular basis for the in-depth study of flavonoid biosynthesis in apricot fruits.
UNASSIGNED: Apricot fruits are rich in flavonoid compounds, and in this study, we performed a combined metabolomic and transcriptomic analysis of orange flesh (JN) and white flesh (ZS) apricot fruits.
UNASSIGNED: A total of 222 differentially accumulated flavonoids (DAFs) and 15855 differentially expressed genes (DEGs) involved in flavonoid biosynthesis were identified. The biosynthesis of flavonoids in apricot fruit may be regulated by 17 enzyme-encoding genes, namely PAL (2), 4CL (9), C4H (1), HCT (15), C3\'H (4), CHS (2), CHI (3), F3H (1), F3\'H (CYP75B1) (2), F3\'5\'H (4), DFR (4), LAR (1), FLS (3), ANS (9), ANR (2), UGT79B1 (6) and CYP81E (2). A structural gene-transcription factor (TF) correlation analysis yielded 3 TFs (2 bHLH, 1 MYB) highly correlated with 2 structural genes. In addition, we obtained 26 candidate genes involved in the biosynthesis of 8 differentially accumulated flavonoids metabolites in ZS by weighted gene coexpression network analysis. The candidate genes and transcription factors identified in this study will provide a highly valuable molecular basis for the in-depth study of flavonoid biosynthesis in apricot fruits.
摘要：
■黄酮类化合物，作为植物的次生代谢产物，在许多生物过程和对环境因素的反应中起着重要作用。
■杏果富含黄酮类化合物，在这项研究中，我们对橙肉（JN）和白肉（ZS）杏果进行了代谢组学和转录组学分析。
■总共鉴定了222个差异积累的类黄酮（DAF）和15855个差异表达的基因（DEGs）参与类黄酮生物合成。杏果中黄酮类化合物的生物合成可能受17个酶编码基因的调控,即PAL(2)，4CL(9),C4H(1),HCT(15),C3\'H(4)，CHS(2),CHI(3),F3H(1),F3\'H(CYP75B1)(2)，F3\'5\'H(4),DFR(4),LAR(1),FLS(3),ANS(9),ANR(2),UGT79B1(6)和CYP81E(2)。结构基因-转录因子（TF）相关分析产生了3个TFs（2bHLH，1MYB）与2个结构基因高度相关。此外,通过加权基因共表达网络分析，我们获得了参与ZS中8种差异积累的类黄酮代谢产物生物合成的26个候选基因。本研究确定的候选基因和转录因子将为深入研究杏果中黄酮类化合物的生物合成提供有价值的分子基础。
■杏果富含黄酮类化合物，在这项研究中，我们对橙肉（JN）和白肉（ZS）杏果进行了代谢组学和转录组学分析。
■总共鉴定了222个差异积累的类黄酮（DAF）和15855个差异表达的基因（DEGs）参与类黄酮生物合成。杏果中黄酮类化合物的生物合成可能受17个酶编码基因的调控,即PAL(2)，4CL(9),C4H(1),HCT(15),C3\'H(4)，CHS(2),CHI(3),F3H(1),F3\'H(CYP75B1)(2)，F3\'5\'H(4),DFR(4),LAR(1),FLS(3),ANS(9),ANR(2),UGT79B1(6)和CYP81E(2)。结构基因-转录因子（TF）相关分析产生了3个TFs（2bHLH，1MYB）与2个结构基因高度相关。此外,通过加权基因共表达网络分析，我们获得了参与ZS中8种差异积累的类黄酮代谢产物生物合成的26个候选基因。本研究确定的候选基因和转录因子将为深入研究杏果中黄酮类化合物的生物合成提供有价值的分子基础。
