Abstract：
UNASSIGNED: Interleukin-10 (IL-10) and tumor necrosis factor-alpha (TNF-α) genes contribute to oncogenesis. We evaluated the influence of the IL-10 (G1082A) and TNF-α (G308A) polymorphisms on the prognosis and outcomes of Egyptian patients with acute lymphoblastic leukemia (ALL).
UNASSIGNED: We investigated 64 children and 76 adults with ALL, between 2016 and 2019, for the IL-10 (G1082A) and TNF-α (G308A) polymorphisms using allele-specific polymerase chain reaction and polymerase chain reaction-restriction fragment length polymorphism. Survival analyses were performed using the Kaplan-Meier estimator and the log-rank test.
UNASSIGNED: In children with ALL, the A allele of TNF-α and IL-10 polymorphisms was associated with older age (P = 0.04 and 0.03), more extramedullary disease (P = 0.02 and 0.001), positive breakpoint cluster region-Abelson (BCR-ABL) rearrangement (p190; P = 0.04 and 0.001), and more relapse (P = 0.002). The IL-10 GG genotype was associated with higher overall survival in children (P = 0.026). Adults carrying the TNF-α A allele showed more extramedullary disease (P = 0.009) and relapse (P = 0.003). We also found a higher frequency of IL-10 A allele in adults with older age (P = 0.03), lower hemoglobin level (P = 0.04), positive BCR-ABL rearrangement (P = 0.001), more extramedullary disease (P = 0.001), more relapse (P = 0.002), and a longer time for the first complete remission (P = 0.003).
UNASSIGNED: A possible association exists between the A allele of IL-10 and TNF-α polymorphisms and poor prognosis in Egyptian patients with ALL, while the IL-10 GG genotype may be associated with better survival in children with ALL.
UNASSIGNED: We investigated 64 children and 76 adults with ALL, between 2016 and 2019, for the IL-10 (G1082A) and TNF-α (G308A) polymorphisms using allele-specific polymerase chain reaction and polymerase chain reaction-restriction fragment length polymorphism. Survival analyses were performed using the Kaplan-Meier estimator and the log-rank test.
UNASSIGNED: In children with ALL, the A allele of TNF-α and IL-10 polymorphisms was associated with older age (P = 0.04 and 0.03), more extramedullary disease (P = 0.02 and 0.001), positive breakpoint cluster region-Abelson (BCR-ABL) rearrangement (p190; P = 0.04 and 0.001), and more relapse (P = 0.002). The IL-10 GG genotype was associated with higher overall survival in children (P = 0.026). Adults carrying the TNF-α A allele showed more extramedullary disease (P = 0.009) and relapse (P = 0.003). We also found a higher frequency of IL-10 A allele in adults with older age (P = 0.03), lower hemoglobin level (P = 0.04), positive BCR-ABL rearrangement (P = 0.001), more extramedullary disease (P = 0.001), more relapse (P = 0.002), and a longer time for the first complete remission (P = 0.003).
UNASSIGNED: A possible association exists between the A allele of IL-10 and TNF-α polymorphisms and poor prognosis in Egyptian patients with ALL, while the IL-10 GG genotype may be associated with better survival in children with ALL.
摘要：
■白细胞介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)基因有助于肿瘤发生。我们评估了IL-10（G1082A）和TNF-α（G308A）多态性对埃及急性淋巴细胞白血病（ALL）患者预后和预后的影响。
■我们调查了64名儿童和76名成年人，在2016年至2019年之间，使用等位基因特异性聚合酶链反应和聚合酶链反应-限制性片段长度多态性对IL-10（G1082A）和TNF-α（G308A）多态性进行了研究。使用Kaplan-Meier估计器和对数秩检验进行生存分析。
■在患有ALL的儿童中，TNF-α和IL-10多态性的A等位基因与年龄相关(P=0.04和0.03),更多的髓外疾病（P=0.02和0.001），正断点簇区-Abelson（BCR-ABL）重排（p190；P=0.04和0.001），和更多的复发（P=0.002）。IL-10GG基因型与较高的儿童总生存率相关(P=0.026)。携带TNF-αA等位基因的成年人表现出更多的髓外疾病（P=0.009）和复发（P=0.003）。我们还发现，在年龄较大的成年人中，IL-10A等位基因的频率更高（P=0.03）。血红蛋白水平较低（P=0.04），BCR-ABL重排阳性（P=0.001），更多的髓外疾病（P=0.001），复发更多（P=0.002），首次完全缓解时间较长(P=0.003)。
■IL-10和TNF-α多态性的A等位基因与埃及ALL患者的不良预后之间可能存在关联，而IL-10GG基因型可能与ALL患儿更好的生存率相关。
■我们调查了64名儿童和76名成年人，在2016年至2019年之间，使用等位基因特异性聚合酶链反应和聚合酶链反应-限制性片段长度多态性对IL-10（G1082A）和TNF-α（G308A）多态性进行了研究。使用Kaplan-Meier估计器和对数秩检验进行生存分析。
■在患有ALL的儿童中，TNF-α和IL-10多态性的A等位基因与年龄相关(P=0.04和0.03),更多的髓外疾病（P=0.02和0.001），正断点簇区-Abelson（BCR-ABL）重排（p190；P=0.04和0.001），和更多的复发（P=0.002）。IL-10GG基因型与较高的儿童总生存率相关(P=0.026)。携带TNF-αA等位基因的成年人表现出更多的髓外疾病（P=0.009）和复发（P=0.003）。我们还发现，在年龄较大的成年人中，IL-10A等位基因的频率更高（P=0.03）。血红蛋白水平较低（P=0.04），BCR-ABL重排阳性（P=0.001），更多的髓外疾病（P=0.001），复发更多（P=0.002），首次完全缓解时间较长(P=0.003)。
■IL-10和TNF-α多态性的A等位基因与埃及ALL患者的不良预后之间可能存在关联，而IL-10GG基因型可能与ALL患儿更好的生存率相关。
