PDF(Pubmed)
Abstract：
X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryocrystallography has never been performed in Türkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, İstanbul, Türkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis.
摘要：
X射线晶体学是一种强大而强大的结构生物学技术，可提供生物大分子的高分辨率原子结构。科学家使用这种技术来解开生物大分子的机械和结构细节(例如，蛋白质，核酸,蛋白质复合物，蛋白质-核酸复合物，或大型生物隔室)。自成立以来,由于缺乏单晶X射线衍射仪，从未在Türkiye进行过单晶低温晶体学。最近在健康科学大学建立的X射线衍射设施，伊斯坦布尔,Türkiye将使土耳其和国际研究人员能够轻松地对单晶生物大分子进行高分辨率结构分析。这里,我们描述了最先进的家用X射线的技术和实践前景，使用溶菌酶作为模型蛋白质。本文中描述的方法和实践可以应用于任何用于结构研究的生物样品。因此,本文将为从样品制备到数据分析提供有价值的实用指南。
