Abstract：
In this study, copper nanozyme (CuNZs) possess good laccase-like activity were synthesized by grinding method with cupric chloride dihydrate as copper source, sodium borohydride as reducing agent and β-cyclodextrin as protective agent. The CuNZs can oxidize colorless 2,4-dinitrophenol (2,4-DP) to red product. When oxytetracycline (OTC) was added to the above three solutions, the color changed from red to orange and the absorbance increased again, indicating that OTC was also an affinity substrate for CuNZs. When CuNZs was mixed with OTC alone, the color changed from colorless to yellow, and the absorption intensity was related to OTC concentration. It has good selectivity and sensitivity, and had a good linear response to the concentration of OTC in the range of 50-500 μM, and the limit of detection was 0.148 μM. Thus, a fast and simple colorimetric assay for the determination of OTC was established by using the laccase-like activity of CuNZs, and it was applied successfully to detect OTC in food samples.
摘要：
在这项研究中,以二水合氯化铜为铜源,采用研磨法合成了具有良好漆酶样活性的铜纳米酶,硼氢化钠为还原剂,β-环糊精为保护剂。CuNZ可以将无色的2,4-二硝基苯酚(2,4-DP)氧化为红色产物。当将土霉素（OTC）添加到上述三种溶液中时，颜色从红色变为橙色，吸光度再次增加，表明OTC也是CuNZs的亲和底物。当CuNZs与OTC单独混合时，颜色从无色变成黄色，吸收强度与OTC浓度有关。它具有良好的选择性和灵敏度，在50-500μM范围内对OTC的浓度具有良好的线性响应,检测限为0.148μM。因此，利用CuNZs的漆酶样活性，建立了一种快速简单的测定OTC的比色法，并成功应用于食品样品中OTC的检测。
