Abstract：
Selenium (Se) is an essential trace element that is involved in several pathophysiological functions. The relationship of Se with cardiovascular disease remains inconclusive, especially regarding the role of different selenospecies.
The present study assessed the levels of Se distribution in plasma selenoproteins, namely glutathione peroxidase 3 (GPx3), selenoprotein P (SelP) and selenoalbumin (SeAlb) and total Se in selenoproteins in relation to 10-year cardiovascular risk in the ATTICA prospective study.
A sub-sample from the ATTICA Study\'s database, consisting of 278 subjects (114 women and 164 men) with data on Se and selenoproteins levels, was considered. SeGPx3, SelP, and SeAlb in human plasma were simultaneously determined by high-performance liquid chromatography (HPLC) coupled with inductively coupled plasma mass spectrometry (ICP-MS) at baseline. The duration of the follow-up was 8.74 ±2.36 years (mean± standard deviation) and cardiovascular outcomes were recorded. Cox proportional hazards models were applied with total Se or selenoprotein Se as independent variables adjusted for several covariates.
Total Se in selenoproteins was positively related to 10-year relative risk of cardiovascular disease (Hazard Ratios of 3rd vs 2nd tertile 10.02, 95% CI:1.15, 92.34). Subjects with high Se but low SeGPx3, as identified by discordant percentiles in the distribution of SeGPx3 and Se, had a higher cardiovascular risk.
The differentiated effects of circulating selenoproteins on cardiovascular disease risk in the present study, suggest the importance of redox regulation by specific selenoproteins.
The present study assessed the levels of Se distribution in plasma selenoproteins, namely glutathione peroxidase 3 (GPx3), selenoprotein P (SelP) and selenoalbumin (SeAlb) and total Se in selenoproteins in relation to 10-year cardiovascular risk in the ATTICA prospective study.
A sub-sample from the ATTICA Study\'s database, consisting of 278 subjects (114 women and 164 men) with data on Se and selenoproteins levels, was considered. SeGPx3, SelP, and SeAlb in human plasma were simultaneously determined by high-performance liquid chromatography (HPLC) coupled with inductively coupled plasma mass spectrometry (ICP-MS) at baseline. The duration of the follow-up was 8.74 ±2.36 years (mean± standard deviation) and cardiovascular outcomes were recorded. Cox proportional hazards models were applied with total Se or selenoprotein Se as independent variables adjusted for several covariates.
Total Se in selenoproteins was positively related to 10-year relative risk of cardiovascular disease (Hazard Ratios of 3rd vs 2nd tertile 10.02, 95% CI:1.15, 92.34). Subjects with high Se but low SeGPx3, as identified by discordant percentiles in the distribution of SeGPx3 and Se, had a higher cardiovascular risk.
The differentiated effects of circulating selenoproteins on cardiovascular disease risk in the present study, suggest the importance of redox regulation by specific selenoproteins.
摘要：
背景：硒（Se）是一种必需的微量元素，与几种病理生理功能有关。硒与心血管疾病的关系仍然没有定论，特别是关于不同硒种的作用。
目的：本研究评估了血浆硒蛋白中硒的分布水平，即谷胱甘肽过氧化物酶3（GPx3），ATTICA前瞻性研究中硒蛋白P（SelP）和硒蛋白（SeAlb）和硒蛋白中总硒与10年心血管风险的关系。
方法：来自ATTICA研究数据库的子样本，由278名受试者（114名女性和164名男性）组成，具有硒和硒蛋白水平的数据，被考虑。SeGPx3,SelP,同时采用高效液相色谱(HPLC)结合电感耦合等离子体质谱(ICP-MS)测定人血浆中的SeAlb。随访时间为8.74±2.36年（平均值±标准差），记录心血管结局。Cox比例风险模型以总Se或硒蛋白Se作为独立变量，针对几个协变量进行了调整。
结果：硒蛋白中的总硒与心血管疾病的10年相对风险呈正相关（第三与第二三元组的危险比10.02，95％CI：1.15，92.34）。具有高Se但低SeGPx3的受试者，如通过SeGPx3和Se分布中的不一致百分位数确定的，有更高的心血管风险。
结论：本研究中循环硒蛋白对心血管疾病风险的不同影响，提示特定硒蛋白对氧化还原调节的重要性。
目的：本研究评估了血浆硒蛋白中硒的分布水平，即谷胱甘肽过氧化物酶3（GPx3），ATTICA前瞻性研究中硒蛋白P（SelP）和硒蛋白（SeAlb）和硒蛋白中总硒与10年心血管风险的关系。
方法：来自ATTICA研究数据库的子样本，由278名受试者（114名女性和164名男性）组成，具有硒和硒蛋白水平的数据，被考虑。SeGPx3,SelP,同时采用高效液相色谱(HPLC)结合电感耦合等离子体质谱(ICP-MS)测定人血浆中的SeAlb。随访时间为8.74±2.36年（平均值±标准差），记录心血管结局。Cox比例风险模型以总Se或硒蛋白Se作为独立变量，针对几个协变量进行了调整。
结果：硒蛋白中的总硒与心血管疾病的10年相对风险呈正相关（第三与第二三元组的危险比10.02，95％CI：1.15，92.34）。具有高Se但低SeGPx3的受试者，如通过SeGPx3和Se分布中的不一致百分位数确定的，有更高的心血管风险。
结论：本研究中循环硒蛋白对心血管疾病风险的不同影响，提示特定硒蛋白对氧化还原调节的重要性。
