Abstract:
Many emerging factors and circumstances urge the need to develop and optimize the detection and quantification techniques of mycotoxins in solid food and feed. The diversity of mycotoxins, which have different properties and affinities, makes the standardization of the analytical procedures and the adoption of a single protocol that covers the attributes of all mycotoxins a tedious or even an impossible mission. Several modifications and improvements have been undergone in order to optimize the performance of these methods including the extraction solvents, the extraction methods, the clean-up procedures, and the analytical techniques. The techniques range from the rapid screening methods, which lack sensitivity and specificity such as TLC, to a spectrum of more advanced protocols, namely, ELISA, HPLC, and GC-MS and LC-MS/MS. This review aims at assessing the current studies related to these analytical techniques of mycotoxins in solid food and feed. It discusses and evaluates, through a critical approach, various sample treatment techniques, and provides an in-depth examination of different mycotoxin detection methods. Furthermore, it includes a comparison of their actual accuracy and a thorough analysis of the observed benefits and drawbacks.
摘要:
许多新出现的因素和情况促使需要开发和优化固体食品和饲料中霉菌毒素的检测和定量技术。真菌毒素的多样性,它们具有不同的属性和亲和力,使分析程序的标准化和采用涵盖所有真菌毒素属性的单一协议是一项乏味甚至不可能的任务。进行了一些修改和改进,以优化这些方法的性能,包括提取溶剂,提取方法,清理程序,和分析技术。技术范围从快速筛选方法,缺乏灵敏度和特异性,如TLC,一系列更先进的协议,即,ELISA,HPLC,GC-MS和LC-MS。这篇综述旨在评估与固体食品和饲料中霉菌毒素的这些分析技术相关的当前研究。它讨论和评估,通过一个关键的方法,各种样品处理技术,并对不同的霉菌毒素检测方法进行了深入的考察。此外,它包括对它们的实际准确性的比较和对观察到的利弊的彻底分析。