PDF(Pubmed)
Abstract：
At present, many infectious pathogens, especially emerging/re-emerging pathogens, exist in the blood of voluntary blood donors and may be transmitted through blood transfusions. However, most of Chinese blood centers only routinely screen for HBV, HCV, HIV, and syphilis. We employed metagenomic next-generation sequencing (mNGS) to investigate the microbiome in healthy voluntary blood donors to help assess blood safety in China by identifying infectious pathogens presented in donations that could lead to transfusion-acquired infections. We collected 10,720 plasma samples from voluntary blood donors from seven blood centers in different cities during 2012-2018 in China. A total of 562 GB of clean data was obtained. By analyzing the sequencing data, it was found that the most commonly identified bacteria found in the healthy blood were Serratia spp. (5.0176%), Pseudomonas spp. (0.6637%), and Burkholderia spp. (0.5544%). The principal eukaryote were Leishmania spp (1.3723%), Toxoplasma gondii (0.6352%), and Candida dubliniensis (0.1848%). Among viruses, Human Parvovirus B19 (B19V) accounts for the highest proportion (0.1490%), followed by Torque teno midi virus (0.0032%) and Torque teno virus (0.0015%). Since that B19V is a non-negligible threat to blood safety, we evaluated the positive samples for B19V tested by mNGS using quantitative polymerase chain reaction, Sanger sequencing, and phylogenetic analysis to achieve a better understanding of B19V in Chinese blood donors. Subsequently, 9 (0.07%) donations were positive for B19V DNA. The quantitative DNA levels ranged from 5.58 × 102 to 7.24 × 104 IU/ml. The phylogenic analyses showed that prevalent genotypes belonged to the B19-1A subtype, which disclosed previously unknown regional variability in the B19V positivity rate. The investigation revealed that many microbes dwell in the blood of healthy donors, including some pathogens that may be dormant in the blood and only cause disease under specific conditions. Thus, investigating the range and nature of potential pathogens in the qualified donations provided a framework for targeted interventions to help prevent emerging and re-emerging infectious diseases.
摘要：
目前,许多传染性病原体，尤其是新出现/重新出现的病原体，存在于无偿献血者的血液中，可能通过输血传播。然而,大多数中国血液中心仅常规筛查HBV，HCV,艾滋病毒,还有梅毒.我们使用宏基因组下一代测序（mNGS）来调查健康无偿献血者的微生物组，以通过识别捐赠中可能导致输血获得性感染的感染性病原体来帮助评估中国的血液安全性。我们在2012-2018年期间从中国不同城市的7个血液中心的无偿献血者收集了10,720份血浆样本。总共获得了562GB的干净数据。通过分析测序数据，发现健康血液中最常见的细菌是沙雷氏菌。（5.0176%），假单胞菌属。（0.6637%），和伯克霍尔德氏菌.(0.5544%)。主要真核生物是利什曼原虫（1.3723％），弓形虫（0.632%），和念珠菌（0.1848%）。在病毒中，人细小病毒B19(B19V)所占比例最高(0.1490%),其次是Torquetenomidi病毒(0.0032%)和Torqueteno病毒(0.0015%)。由于B19V对血液安全是不可忽视的威胁，我们使用定量聚合酶链反应评估了mNGS检测的B19V阳性样品，桑格测序,和系统发育分析，以更好地了解中国献血者的B19V。随后，9（0.07%）的B19VDNA捐赠为阳性。定量DNA水平范围为5.58×102至7.24×104IU/ml。系统发育分析表明，流行基因型属于B19-1A亚型，这揭示了B19V阳性率以前未知的区域变异性。调查显示，许多微生物居住在健康捐赠者的血液中，包括一些可能在血液中休眠并且仅在特定条件下引起疾病的病原体。因此，调查合格捐赠中潜在病原体的范围和性质为有针对性的干预措施提供了框架，以帮助预防新出现和再次出现的传染病。
