关键词: Dental aerosols biofilms communicable disease control dental equipment microbial load

来  源:   DOI:10.1016/j.jfscie.2021.100003   PDF(Pubmed)

Abstract:
UNASSIGNED: The COVID-19 pandemic has heightened the awareness of a common hazard encountered in the dental clinic: aerosol transmission of pathogens. Treatment of sources of infection before or during dental procedures is one means of decreasing pathogen load and aerosol transmission.
UNASSIGNED: An ultrasonic scaler supplied with aqueous ozone was used to examine the effect of its viability on planktonic cultures and biofilms formed by 2 model bacteria: Rothia mucilaginosa and Escherichia coli.
UNASSIGNED: Both organisms showed susceptibility to aqueous ozone alone (97% and 99.5% lethality, respectively). When combined with manual scaling using an ultrasonic scaler, a greater than 99% reduction in colony-forming units (CFUs)/mL could be reached with an aqueous ozone concentration of approximately 2 mg/L (R. mucilaginosa) or 0.75 mg/L (E. coli) after 5 through 6 seconds of scaling.
UNASSIGNED: Aqueous ozone coupled with ultrasonic scaling exhibited a higher efficiency of microbial kill than either method used alone. Both gram-positive and gram-negative species were affected by this treatment. Studies on other oral microbiota constituents, including fungi and viruses, will provide information on the efficacy of this method on a greater biological scale. Studies to verify concomitant reduction of microbial load in dispersed aerosols in clinical settings should be completed to support practical applications of this treatment.
摘要:
COVID-19大流行提高了人们对牙科诊所遇到的常见危害的认识:病原体的气溶胶传播。在牙科手术之前或期间治疗感染源是减少病原体负荷和气溶胶传播的一种手段。
使用提供有臭氧水溶液的超声波洁牙机来检查其生存力对浮游培养物和由2种模型细菌形成的生物膜的影响:红藻和大肠杆菌。
两种生物均显示出对臭氧水溶液的敏感性(分别为97%和99.5%的致死率,分别)。当结合使用超声波洁牙机手动缩放时,在约2mg/L的臭氧水溶液浓度下,菌落形成单位(CFUs)/mL可以降低99%以上(R。粘胶)或0.75mg/L(E.大肠杆菌)在5到6秒的结垢后。
与超声波结垢相结合的臭氧水溶液比单独使用的任何一种方法都显示出更高的微生物杀灭效率。革兰氏阳性和革兰氏阴性物种都受到这种处理的影响。其他口腔微生物群成分的研究,包括真菌和病毒,将在更大的生物尺度上提供有关此方法功效的信息。应完成验证临床环境中分散气溶胶中微生物负荷伴随减少的研究,以支持该治疗的实际应用。
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