Abstract：
Challenges and pitfalls in the application of diethyldithiocarbamate derivatization for LC analysis of cisplatin and oxaliplatin, as well as the suitability of this method for different biological matrices with implications for use in routine practice have been identified. The LC of platinum drugs presents a significant challenge. They are polar compounds with poor retention on reverse phase packings. Cisplatin also exhibits poor absorption in UV and ionization in mass spectrometry. Therefore, we developed and optimized a derivatization approach for the LC analysis of total platinum in plasma, plasma ultrafiltrate, peritoneal fluid, and urine. Derivatization in urine proved to be difficult due to the complexity of the matrix, and extended testing was required. Our results highlight the important issues affecting the efficiency, reliability, and suitability of platinum drug derivatization. Although precolumn derivatization is less selective than its postcolumn counterpart, the application of precolumn derivatization is a simple, rapid, and universal approach for the determination of platinum drugs by HPLC. One of its major advantages is that it allows a more affordable analysis using UV detection without the need for additional high-end instrumentation such as a MS detector.
摘要：
二乙基二硫代氨基甲酸酯衍生化在顺铂和奥沙利铂LC分析中的挑战和陷阱，以及该方法对不同的生物基质的适用性，已经确定了在常规实践中使用的意义。铂药物的LC提出了重大挑战。它们是极性化合物，在反相填料上的保留率差。顺铂还表现出在UV中的差的吸收和在质谱中的电离。因此,我们开发并优化了一种衍生化方法，用于等离子体中总铂的LC分析，血浆超滤液，腹膜液,还有尿液.由于基质的复杂性，尿液中的衍生被证明是困难的，和延长测试是必需的。我们的结果突出了影响效率的重要问题，可靠性,和铂类药物衍生化的适用性。尽管柱前衍生的选择性不如柱后衍生，柱前衍生化的应用是一个简单的，快速,高效液相色谱法测定铂类药物的通用方法。其主要优点之一是，它允许使用UV检测进行更实惠的分析，而无需额外的高端仪器，如MS检测器。
