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Abstract：
African swine fever virus (ASFV) is the etiological agent of a haemorrhagic disease that threatens the global pig industry. There is an urgency to develop a safe and efficient vaccine, but the knowledge of the immune-pathogenetic mechanisms behind ASFV infection is still very limited. In this paper, we evaluated the haematological and immunological parameters of domestic pigs vaccinated with the ASFV Lv17/WB/Rie1 strain or its derived mutant Lv17/WB/Rie1/d110-11L and then challenged with virulent Armenia/07 ASFV. Circulating levels of C-reactive protein (CRP), 13 key cytokines and 11 haematological parameters were evaluated throughout the study. Lv17/WB/Rie1 triggered an inflammatory response, with increased levels of CRP and pro-inflammatory cytokines, and induced lymphopenia, thrombocytopenia and a decline in red blood cell (RBC) parameters, although this was transitory. Lv17/WB/Rie1/d110-11L triggered only transitory thrombocytopenia and a mild inflammatory reaction, with no increase in serum levels of pro-inflammatory cytokines, but it raised IL-1Ra levels. Both strains counteracted several adverse reactions elicited by virulent challenge, like thrombocytopenia, a decline in RBC parameters, and inflammation. Within this paper, we provided a deep portrayal of the impact of diverse ASFV strains on the domestic pig\'s immune system. A better understanding of these immune-pathological mechanisms would help to design suitable vaccines against this disease.
摘要：
非洲猪瘟病毒（ASFV）是威胁全球养猪业的出血性疾病的病原体。迫切需要开发一种安全有效的疫苗，但是对ASFV感染背后的免疫致病机制的了解仍然非常有限。在本文中,我们评估了接种了ASFVLv17/WB/Rie1菌株或其衍生的突变体Lv17/WB/Rie1/d110-11L，然后用强毒亚美尼亚/07ASFV攻击的家猪的血液学和免疫学参数。C反应蛋白（CRP）的循环水平，在整个研究中评估了13种关键细胞因子和11种血液学参数。Lv17/WB/Rie1引发炎症反应，随着CRP和促炎细胞因子水平的增加，并诱导淋巴细胞减少，血小板减少和红细胞（RBC）参数下降，虽然这是暂时的。Lv17/WB/Rie1/d110-11L仅引发短暂性血小板减少症和轻度炎症反应，血清促炎细胞因子水平没有增加，但它提高了IL-1Ra水平。两种菌株都抵消了毒力攻击引起的几种不良反应，比如血小板减少症,红细胞参数的下降，和炎症。在这篇论文中，我们提供了不同的ASFV毒株对家猪免疫系统的影响的深度描述。更好地了解这些免疫病理机制将有助于设计针对这种疾病的合适疫苗。
