PDF(Pubmed)
Abstract：
In this study, we examined and compared two different lipid-based nanosystems (LBNs), namely Transferosomes (TFs) and Monoolein Aqueous Dispersions (MADs), as delivery systems for the topical application of Ferulic Acid (FA), an antioxidant molecule derived from natural sources. Our results, as demonstrated through Franz-cell experiments, indicate that the LBNs produced with poloxamer 188 in their composition create a multilamellar system. This system effectively controls the release of the drug. Nonetheless, we found that the type of non-ionic surfactant can impact the drug release rate. Regarding FA diffusion from the MAD, this showed a lower diffusion rate compared with the TF. In terms of an in vivo application, patch tests revealed that all LBN formulations tested were safe when applied under occlusive conditions for 48 h. Additionally, human skin biopsies were used to determine whether FA-containing formulations could influence skin tissue morphology or provide protection against O3 exposure. Analyses suggest that treatment with TFs composed of poloxamer 188 and MAD formulations might protect against structural skin damage (as observed in hematoxylin/eosin staining) and the development of an oxidative environment (as indicated by 4-hyroxinonenal (4HNE) expression levels) induced by O3 exposure. In contrast, formulations without the active ingredient did not offer protection against the detrimental effects of O3 exposure.Inizio modulo.
摘要：
在这项研究中,我们检查并比较了两种不同的基于脂质的纳米系统（LBN），即转铁蛋白体（TFs）和单油酸甘油酯水分散体（MAD），作为局部应用阿魏酸（FA）的输送系统，一种天然来源的抗氧化剂分子。我们的结果,正如弗朗兹细胞实验所证明的，表明用泊洛沙姆188在其组成中产生的LBN产生多层系统。该系统有效地控制药物的释放。尽管如此，我们发现非离子表面活性剂的类型可以影响药物的释放速率。关于FA从MAD的扩散，这显示了与TF相比更低的扩散速率。就体内应用而言，斑贴试验表明，当在闭塞条件下应用48小时时，所有测试的LBN制剂都是安全的。此外，人类皮肤活检用于确定含FA的制剂是否可以影响皮肤组织形态或提供针对O3暴露的保护。分析表明，用泊洛沙姆188和MAD制剂组成的TF治疗可能会防止结构性皮肤损伤（如在苏木精/伊红染色中观察到的）和氧化环境的发展（如4-hyroxinonenal（4HNE）表达水平所示）由O3暴露诱导。相比之下,不含活性成分的制剂不能提供对O3暴露有害影响的保护。Inizio模。
