PDF(Pubmed)
Abstract：
The metabolic profiles of human feces are influenced by various genetic and environmental factors, which makes feces an attractive biosample for numerous applications, including the early detection of gut diseases. However, feces is complex, heterogeneous, and dynamic with a significant live bacterial biomass. With such challenges, stool metabolomics has been understudied compared to other biospecimens, and there is a current lack of consensus on methods to collect, prepare, and analyze feces. One of the critical steps required to accelerate the field is having a metabolomics stool reference material available. Fecal samples are generally presented in two major forms: fecal water and lyophilized feces. In this study, two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC×GC-TOFMS) was used as an analytical platform to characterize pooled human feces, provided by the National Institute of Standards and Technology (NIST) as Research-Grade Test Materials. The collected fecal samples were derived from eight healthy individuals with two different diets: vegans and omnivores, matched by age, sex, and body mass index (BMI), and stored as fecal water and lyophilized feces. Various data analysis strategies were presented to determine the differences in the fecal metabolomic profiles. The results indicate that the sample storage condition has a major influence on the metabolic profiles of feces such that the impact from storage surpasses the metabolic differences from the diet types. The findings of the current study would contribute towards the development of a stool reference material.
摘要：
人类粪便的代谢谱受各种遗传和环境因素的影响，这使得粪便成为许多应用的有吸引力的生物样本，包括早期发现肠道疾病。然而,粪便很复杂,异质，并具有显著的活细菌生物量。面对这样的挑战,与其他生物样本相比，粪便代谢组学研究不足，目前在收集方法上缺乏共识，准备,分析粪便.加速该领域所需的关键步骤之一是提供代谢组学粪便参考材料。粪便样品通常以两种主要形式存在:粪便水和冻干粪便。在这项研究中,二维气相色谱结合飞行时间质谱（GC×GC-TOFMS）用作分析平台，以表征合并的人类粪便，由国家标准与技术研究所（NIST）提供，作为研究级测试材料。收集的粪便样本来自八个健康个体，他们有两种不同的饮食：素食主义者和杂食动物，与年龄相匹配,性别,和体重指数（BMI），并作为粪便水和冻干粪便储存。提出了各种数据分析策略来确定粪便代谢组学特征的差异。结果表明,样品储存条件对粪便的代谢谱具有主要影响,使得来自储存的影响超过与饮食类型的代谢差异。当前研究的结果将有助于粪便参考材料的开发。
