PDF(Pubmed)
Abstract：
Cotton is a valuable cash crop in many countries. Cotton fiber is a trichome that develops from a single epidermal cell and serves as an excellent model for understanding cell differentiation and other life processes. Alternative splicing (AS) of genes is a common post-transcriptional regulatory process in plants that is essential for plant growth and development. The process of AS during cotton fiber formation, on the other hand, is mainly unknown. A substantial number of multi-exon genes were discovered to be alternatively spliced during cotton fiber formation in this study, accounting for 23.31% of the total number of genes in Gossypium hirsutum. Retention intron (RI) is not necessarily the most common AS type, indicating that AS genes and processes during fiber development are very temporal and tissue-specific. When compared to fiber samples, AS is more prevalent at the fiber initiation stages and in the ovule, indicating that development stages and tissues use different AS strategies. Genes involved in fiber development have gone through stage-specific AS, demonstrating that AS regulates cotton fiber development. Furthermore, AS can be regulated by trans-regulation elements such as splicing factor and cis-regulation elements such as gene length, exon numbers, and GC content, particularly at exon-intron junction sites. Our findings also suggest that increased DNA methylation may aid in the efficiency of AS, and that gene body methylation is key in AS control. Finally, our research will provide useful information about the roles of AS during the cotton fiber development process.
摘要：
在许多国家，棉花是一种有价值的经济作物。棉纤维是从单个表皮细胞发育而来的毛状体，是理解细胞分化和其他生命过程的极好模型。基因的可变剪接（AS）是植物中常见的转录后调控过程，对植物的生长和发育至关重要。棉纤维形成过程中的AS，另一方面,主要是未知。在这项研究中，发现了大量的多外显子基因在棉纤维形成过程中被交替剪接。占陆地棉基因总数的23.31%。保留内含子（RI）不一定是最常见的AS类型，表明纤维发育过程中的AS基因和过程是非常时间和组织特异性的。与纤维样品相比，AS在纤维起始阶段和胚珠中更为普遍，表明发育阶段和组织使用不同的AS策略。参与纤维发育的基因已经经历了阶段特异性AS，证明AS调节棉纤维发育。此外，AS可以通过反式调节元件如剪接因子和顺式调节元件如基因长度来调节,外显子数字,和GC含量，特别是在外显子-内含子连接位点。我们的发现还表明，增加的DNA甲基化可能有助于AS的效率，基因体甲基化是AS控制的关键。最后,我们的研究将为棉纤维发育过程中AS的作用提供有用的信息。
