PDF(Pubmed)
Abstract：
The treatment of chondral and osteochondral defects is challenging. These types of lesions are painful and progress to osteoarthritis over time. Tissue engineering offers tools to address this unmet medical need. The use of an autologous cartilage construct consisting of hyaline cartilage chips embedded in plasma rich in growth factors (PRGF) has been proposed as a therapeutic alternative. The purpose of this study was to dig into the potential mechanisms behind the in vitro remodelling process that might explain the clinical success of this technique and facilitate its optimisation. Chondrocyte viability and cellular behaviour over eight weeks of in vitro culture, type II collagen synthesis, the dual delivery of growth factors by hyaline cartilage and PRGF matrix, and the ultrastructure of the construct and its remodelling were characterised. The main finding of this research is that the cartilage fragments embedded in the three-dimensional PRGF scaffold contain viable chondrocytes that are able to migrate into the fibrin network, proliferate and synthesise extracellular matrix after the second week of in vitro culture. The characterization of this three-dimensional matrix is key to unravelling the molecular kinetics responsible for its efficacy.
摘要：
软骨和骨软骨缺损的治疗具有挑战性。这些类型的病变是疼痛的，并且随着时间的推移发展为骨关节炎。组织工程提供了解决这种未满足的医疗需求的工具。已提出使用由嵌入富含生长因子的血浆（PRGF）中的透明软骨芯片组成的自体软骨构建体作为治疗替代方案。这项研究的目的是深入研究体外重塑过程背后的潜在机制，这些机制可能解释该技术的临床成功并促进其优化。体外培养八周的软骨细胞活力和细胞行为，II型胶原蛋白合成，透明软骨和PRGF基质双重传递生长因子，并对结构的超微结构及其重塑进行了表征。这项研究的主要发现是，嵌入三维PRGF支架中的软骨碎片含有能够迁移到纤维蛋白网络中的活软骨细胞，体外培养第二周后增殖和合成细胞外基质。该三维基质的表征是解开负责其功效的分子动力学的关键。
