PDF(Pubmed)
Abstract：
Caring for patients with Crohn\'s disease (CD) is a serious challenge in modern medicine. The increasing incidence of CD among adolescents and the severe course of the disease create the need for new methods of diagnosis and therapy. Endogenous opioids are a group of low molecular weight chemical compounds with analgesic and anti-inflammatory properties. Endorphins, enkephalins, and dynorphins may have potentially beneficial effects on the course of CD. Previous research data on this topic are inconsistent. Some authors have reported an increase in the concentration of leukocytes during the course of inflammatory bowel disease (IBD) while others have described a downward trend, explained by DPP-IV enzyme activity. Even fewer data are available on plasma endo-opioid level. There is also a lack of comprehensive studies that have assessed the endo-opioid system in patients with IBD. Therefore, the objective of this study was to measure the serum concentrations of human β-endorphin, human proenkephalin (A), and human big dynorphin in CD patients in the acute phase of the disease, during hospital treatment, and in the remission state. All determinations were performed using ELISA kits. The results of our study showed that the concentrations of all the tested endo-opioids, especially β-endorphin and proenkephalin (A), were reduced in adolescents with CD compared to those in the healthy control group, during the acute phase of the disease, and in the remission state. Modulation of the endogenous opioid system and the use of selective nonnarcotic agonists of opioid receptors seems to be promising goals in the future treatment of CD.
摘要：
照顾克罗恩病(CD)患者是现代医学面临的严峻挑战。青少年中CD发病率的增加和疾病的严重进程产生了对新的诊断和治疗方法的需要。内源性阿片类药物是一组具有镇痛和抗炎特性的低分子量化合物。内啡肽,脑啡肽,强啡肽可能对CD的病程有潜在的有益作用。以前关于这一主题的研究数据不一致。一些作者报道了炎症性肠病（IBD）过程中白细胞浓度的增加，而另一些作者则描述了下降趋势。由DPP-IV酶活性解释。关于血浆内阿片类药物水平的数据甚至更少。也缺乏评估IBD患者内阿片系统的综合研究。因此,这项研究的目的是测量人β-内啡肽的血清浓度，人脑啡肽原(A),和人类大强啡肽在疾病急性期的CD患者中，在医院治疗期间，处于缓解状态。使用ELISA试剂盒进行所有测定。我们的研究结果表明，所有测试的阿片类药物的浓度，特别是β-内啡肽和前脑啡肽（A），与健康对照组相比，患有CD的青少年减少，在疾病的急性期，处于缓解状态。内源性阿片系统的调节和阿片受体的选择性非麻醉激动剂的使用似乎是未来CD治疗的有希望的目标。
