PDF(Pubmed)
Abstract：
Callosotomy is an invasive method that is used to study the role of interhemispheric functional connectivity in the brain. This surgical approach is technically demanding to perform in small laboratory animals, such as rodents, due to several methodological challenges. To date, there exist two main approaches for transecting the corpus callosum (CC) in rodents: trephine hole(s) or unilateral craniotomy, which cause damage to the cerebral cortex or the injury of large vessels, and may lead to intracranial hemorrhage and animal death. This study presents an improved surgical approach for complete corpus callosotomy in mice using an interhemispheric approach combined with bilateral and extended craniotomy across the midline. This study demonstrated that bilateral and extended craniotomy provided the visual space required for hemisphere and sinus retraction, thus keeping large blood vessels and surrounding brain structures intact under the surgical microscope using standardized surgical instruments. We also emphasized the importance of good post-operative care leading to an increase in overall animal survival following experimentation. This optimized surgical approach avoids extracallosal tissue and medium- to large-sized cerebral blood vessel damage in mice, which can provide higher study reproducibility/validity among animals when revealing the role of the CC in various neurological pathologies.
摘要：
膀胱切开术是一种侵入性方法，用于研究大脑半球间功能连接的作用。这种手术方法对小型实验动物的手术技术要求很高，如啮齿动物，由于一些方法论上的挑战。迄今为止,在啮齿动物中，有两种主要的横断call体（CC）的方法：环钻孔或单侧开颅术，导致大脑皮层损伤或大血管损伤，并可能导致颅内出血和动物死亡。这项研究提出了一种改进的手术方法，用于使用半球间方法结合中线的双侧和扩展开颅术对小鼠进行完整的骨体切开术。这项研究表明，双侧和扩展开颅手术提供了半球和窦回缩所需的视觉空间，因此，使用标准化的手术器械在手术显微镜下保持大血管和周围大脑结构的完整性。我们还强调了良好的术后护理的重要性，从而提高了实验后动物的总体存活率。这种优化的手术方法避免了小鼠的外组织和中大型脑血管损伤，当揭示CC在各种神经病理学中的作用时，可以在动物之间提供更高的研究可重复性/有效性。
