PDF(Pubmed)
Abstract：
Au decorated with type I collagen (Col) was used as a core material to cross-link with stromal cell-derived factor 1α (SDF1α) in order to investigate biological performance. The Au-based nanoparticles were subjected to physicochemical determination using scanning electron microscopy (SEM), dynamic light scattering (DLS) and ultraviolet-visible (UV-Vis) and Fourier-transform infrared spectroscopy (FTIR). Mesenchymal stem cells (MSCs) were used to evaluate the biocompatibility of this nanoparticle using the MTT assay and measuring reactive oxygen species (ROS) production. Also, the biological effects of the SDF-1α-conjugated nanoparticles (Au-Col-SDF1α) were assessed and the mechanisms were explored. Furthermore, we investigated the cell differentiation-inducing potential of these conjugated nanoparticles on MSCs toward endothelial cells, neurons, osteoblasts and adipocytes. We then ultimately explored the process of cell entry and transportation of the nanoparticles. Using a mouse animal model and retro-orbital sinus injection, we traced in vivo biodistribution to determine the biosafety of the Au-Col-SDF1α nanoparticles. In summary, our results indicate that Au-Col is a promising drug delivery system; it can be used to carry SDF1α to improve MSC therapeutic efficiency.
摘要：
用I型胶原蛋白（Col）修饰的Au用作核心材料，与基质细胞衍生因子1α（SDF1α）交联，以研究生物学性能。使用扫描电子显微镜（SEM）对Au基纳米颗粒进行物理化学测定，动态光散射（DLS）和紫外可见（UV-Vis）和傅里叶变换红外光谱（FTIR）。使用间充质干细胞(MSC)使用MTT测定和测量活性氧(ROS)产生来评估该纳米颗粒的生物相容性。此外，评估了SDF-1α共轭纳米颗粒（Au-Col-SDF1α）的生物学效应，并探讨了其机制。此外，我们研究了这些缀合的纳米颗粒对MSCs向内皮细胞的细胞分化诱导潜力，神经元，成骨细胞和脂肪细胞。然后，我们最终探索了纳米颗粒的细胞进入和运输过程。使用小鼠动物模型和眶后窦注射，我们追踪体内生物分布以确定Au-Col-SDF1α纳米颗粒的生物安全性。总之,我们的结果表明，Au-Col是一种有前途的药物递送系统；它可以用于携带SDF1α以提高MSC的治疗效率。
