PDF(Pubmed)
Abstract：
Few data are available evaluating the stability of direct-fed microbials (DFM) following their inclusion in different feed matrices. Therefore, six Exp. evaluated the recovery of bacilli spores (BOVACILLUSTM; Exp. 1 to 3) and an Enterococcus faecium DFM (LACTIFERM®; Exp. 4 to 6) when included in different feed preparations. The Bacillus-based DFM was included into pelleted feed prepared in different temperatures (75 to 95 °C), whereas both DFM were assessed in premix and milk replacer preparations. Bacillus spores and E. faecium recovery was evaluated through standard methodologies and data were reported as log10 colony forming units/gram of feed. The recovery of Bacillus spores was within the expected range and was not impacted by the temperature of pellet preparation (Exp. 1). Bacilli recovery was also stable up to 12 months in the premix and was not impacted by the temperature of milk replacer preparation. Regarding the Exp. with E. faecium (Exp. 4 to 6), its recoveries in the mineral premix and milk powder did not differ from T0 and were not impacted by the conditions of milk replacer preparation. These data are novel and demonstrate the stability of a Bacillus-based and an E. faecium-based DFM when included in different feed matrices often used in dairy production.
摘要：
在将直接饲喂的微生物(DFM)包含在不同的饲料基质中之后,几乎没有可获得的评估其稳定性的数据。因此,六Exp。评估了杆菌孢子的回收率(BOVACILLUSTM；实验。1至3）和屎肠球菌DFM（LACTIFERM®；Exp。4至6）当包含在不同的饲料制剂中时。将基于芽孢杆菌的DFM包括在不同温度（75至95°C）下制备的粒状饲料中，而两种DFM均在预混物和牛奶替代品制剂中进行了评估。通过标准方法评价芽孢杆菌孢子和屎肠球菌的回收率,并将数据报告为log10菌落形成单位/克饲料。芽孢杆菌孢子的回收率在预期范围内，并且不受颗粒制备温度的影响（Exp。1).在预混物中,芽孢杆菌的回收也稳定达12个月,并且不受牛奶替代品制备温度的影响。关于Exp。与E.屎肠杆菌（实验。4至6），其在矿物质预混物和奶粉中的回收率与T0没有差异，并且不受牛奶替代品制备条件的影响。这些数据是新颖的,并且证明了基于芽孢杆菌和基于屎肠球菌的DFM在被包括在乳制品生产中常用的不同饲料基质中时的稳定性。
