PDF(Pubmed)
Abstract：
Equilibration with an extender is necessary to allow cryopreservation of bovine sperm. The aim of trial 1 was to assess the effect of 24 h versus 4 h equilibration time with three different extenders on sperm quality and to select the preferred extender for each bull. The aim of trial 2 was to investigate the effect of using a 24 h equilibration time with a bull-specific extender on field fertility. For trial 1, three ejaculates each from eight Holstein Friesian breeding bulls were used as the split-sample, including two equilibration times (4 h and 24 h) and three extenders (BioXcell, Triladyl, and OptiXcell). For trial 2, from 5 to 10 ejaculates from the same bulls were collected and treated (split-sample) as BioXcell with 4 h equilibration and either Triladyl or OptiXcell, both with 24 h equilibration. A total of 11,059 straws were used for insemination of cows and heifers. For Triladyl, progressive sperm motility, acrosome defects, and plasma membrane and acrosome integrity improved with a 24 h compared to a 4 h equilibration time. Four bulls each were used with Triladyl and OptiXcell for trial 2. In trial 2, non-return rates did not differ among groups. Therefore, using a 24 h equilibration time might improve in vitro sperm parameters, depending on the extender used. Moreover, it would be possible to change from 4 h to 24 h equilibration time without impairing field fertility.
摘要：
为了冷冻保存牛精子，必须使用扩展器进行平衡。试验1的目的是评估三种不同增量剂的24小时与4小时平衡时间对精子质量的影响，并为每头公牛选择首选的增量剂。试验2的目的是研究使用24小时平衡时间与公牛特异性扩展器对田间生育力的影响。对于试验1，分别来自八只荷斯坦·弗利西亚繁殖公牛的三只射精被用作分割样本，包括两个平衡时间(4小时和24小时)和三个增量剂(BioXcell，Triladyl,和OptiXcell）。对于试验2，收集来自相同公牛的5至10个射精，并以BioXcell进行4小时平衡和Triladyl或OptiXcell处理（分割样品），两者都有24小时平衡。共有11,059根吸管用于奶牛和小母牛的授精。对于Triladyl,进行性精子运动性，顶体缺陷,与4小时的平衡时间相比，质膜和顶体的完整性在24小时内得到了改善。四只公牛分别与Triladyl和OptiXcell一起用于试验2。在试验2中，未返回率在组间没有差异。因此,使用24小时的平衡时间可能会改善体外精子参数，取决于所使用的扩展器。此外，可以将平衡时间从4h更改为24h，而不会损害田间肥力。
