PDF(Pubmed)
Abstract：
BACKGROUND: The symbiosis between the Hawaiian bobtail squid Euprymna scolopes and bacterium Vibrio fischeri serves as a model for investigating the molecular mechanisms that promote the initial formation of animal-bacterial symbioses. Research with this system frequently depends on freshly hatched E. scolopes, but the husbandry factors that promote hatchling production in a mariculture facility remain underreported. Here we report on the reproductive performance of E. scolopes in response to decreased mating frequency.
RESULTS: One animal cohort was maintained in a mariculture facility for 107 days, with females assigned to either a control group (mating once every 14 days) or an experimental group (mating once every 21 days). No differences between the groups were observed in survival, the number of egg clutches laid, or hatchling counts. Each group featured multiple females that were hyper-reproductive, i.e., they generated more than 8 egg clutches while in captivity. Examination of the distributions for daily hatchling counts of individual egg clutches revealed significant variation in the hatching patterns among clutches that was independent of mating frequency. Finally, an assessment of hatchling production showed that 93.5% of total hatchlings produced by the cohort were derived from egg clutches laid within the first 70 days.
CONCLUSIONS: These results suggest a lower mating frequency does not impede hatchling production. Furthermore, the variation in hatchling production among egg clutches provides new insight into the reproductive performance of E. scolopes as a lab animal for microbiology research.
RESULTS: One animal cohort was maintained in a mariculture facility for 107 days, with females assigned to either a control group (mating once every 14 days) or an experimental group (mating once every 21 days). No differences between the groups were observed in survival, the number of egg clutches laid, or hatchling counts. Each group featured multiple females that were hyper-reproductive, i.e., they generated more than 8 egg clutches while in captivity. Examination of the distributions for daily hatchling counts of individual egg clutches revealed significant variation in the hatching patterns among clutches that was independent of mating frequency. Finally, an assessment of hatchling production showed that 93.5% of total hatchlings produced by the cohort were derived from egg clutches laid within the first 70 days.
CONCLUSIONS: These results suggest a lower mating frequency does not impede hatchling production. Furthermore, the variation in hatchling production among egg clutches provides new insight into the reproductive performance of E. scolopes as a lab animal for microbiology research.
摘要：
背景：夏威夷短尾鱿鱼Euprymnacoscoopes与细菌弧菌之间的共生关系可作为研究促进动物-细菌共生初始形成的分子机制的模型。该系统的研究通常取决于刚孵化的大肠杆菌，但是在海产养殖设施中促进孵化生产的畜牧业因素仍然被低估。在这里，我们报告了E.scolines对交配频率降低的反应的繁殖性能。
结果：一个动物队列在海水养殖设施中维持107天，将雌性分配到对照组（每14天交配一次）或实验组（每21天交配一次）。两组之间的生存率没有差异，产卵的数量，或孵化计数。每个组都有多名女性，他们的生殖能力很强，即，他们在被囚禁时产生了8个以上的卵。对单个卵离合器的每日孵化计数分布的检查显示，离合器之间的孵化模式存在显着变化，这与配合频率无关。最后,对孵化生产的评估显示，该队列生产的总孵化中有93.5％来自最初70天内产下的卵。
结论：这些结果表明，较低的交配频率不会阻碍孵化的生产。此外，卵离合器之间孵化产量的变化为大肠杆菌作为微生物学研究的实验动物的繁殖性能提供了新的见解。
结果：一个动物队列在海水养殖设施中维持107天，将雌性分配到对照组（每14天交配一次）或实验组（每21天交配一次）。两组之间的生存率没有差异，产卵的数量，或孵化计数。每个组都有多名女性，他们的生殖能力很强，即，他们在被囚禁时产生了8个以上的卵。对单个卵离合器的每日孵化计数分布的检查显示，离合器之间的孵化模式存在显着变化，这与配合频率无关。最后,对孵化生产的评估显示，该队列生产的总孵化中有93.5％来自最初70天内产下的卵。
结论：这些结果表明，较低的交配频率不会阻碍孵化的生产。此外，卵离合器之间孵化产量的变化为大肠杆菌作为微生物学研究的实验动物的繁殖性能提供了新的见解。
