Abstract：
Tapeworm infections cause insidious and irreversible effects in the infected individuals and some of them have already shown resistance to available drugs. A search for alternative treatment is urgently required. Phenolic compounds are amongst the most researched natural substances for their medicinal use. The present study aims to determine anthelmintic efficacy of two polyphenols Gallic acid and Catechin against the zoonotic rat tapeworm Hymenolepis diminuta. Both compounds are potent anti-oxidants and play major roles in combating pathogens, while their anthelmintic property according to our knowledge is yet to be explored. The parasite model H. diminuta was procured from intestine of infected rats raised in our laboratory. Two sets of parasites were treated in vitro with 5, 10, 20 and 40 mg/ml concentrations of each Gallic Acid and Catechin separately, another set of parasites were treated with standard dose of Praziquantel in RPMI 1640, while still another set of worms were kept in RPMI 1640 at 37 ± 10C with 1% Dimethyl sulfoxide as control. Motility and structural alterations were the parameters assessed for anthelmintic efficacy of the compounds. After paralysis the worms were processed for morphological, histological, and ultrastructural study and observed under light and electron microscope. Dose-dependent efficacy was observed in both compounds. Shrinkage of suckers, deformed proglottids and architectural alteration of the tegument were observed throughout the body of treated parasites compared to control. Although in terms of time taken for paralysis and mortality Gallic acid was more effective than Catechin, the degree of morphological aberrations caused were almost similar, except histological alteration was more in Catechin treated worms than in Gallic acid. Nevertheless, both Gallic acid and Catechin are suggested to possess anthelmintic efficacy besides other health benefits but extended studies are required to compare their efficacy.
摘要：
丝虫感染在受感染的个体中引起阴险和不可逆的影响，其中一些已经显示出对可用药物的抗性。迫切需要寻求替代治疗。酚类化合物是研究最多的天然物质，用于其药用。本研究旨在确定两种多酚没食子酸和儿茶素对人畜共患大鼠tape虫的驱虫功效。这两种化合物都是有效的抗氧化剂，在对抗病原体方面发挥着重要作用。而根据我们的知识，它们的驱虫特性还有待探索。寄生虫模型H.diminuta是从我们实验室饲养的受感染大鼠的肠道中获得的。分别用5、10、20和40mg/ml浓度的没食子酸和儿茶素在体外处理两组寄生虫，另一组寄生虫在RPMI1640中用标准剂量的吡喹酮治疗,而另一组蠕虫在37±10℃的RPMI1640中以1%二甲基亚砜作为对照.运动性和结构改变是评估化合物驱虫功效的参数。瘫痪后，对蠕虫进行了形态学处理，组织学,和超微结构研究，并在光镜和电子显微镜下观察。在两种化合物中均观察到剂量依赖性功效。吸盘的收缩，与对照组相比，在整个接受治疗的寄生虫体内观察到变形的前角体和外皮的结构改变。尽管就瘫痪和死亡率而言，没食子酸比儿茶素更有效，引起的形态畸变程度几乎相似，除了儿茶素治疗的蠕虫的组织学改变比没食子酸更多。然而，除了其他健康益处外，没食子酸和儿茶素都被认为具有驱虫功效，但需要进行更广泛的研究来比较它们的功效。
