PDF(Pubmed)
Abstract：
Chikungunya is a mosquito-borne viral disease caused by the chikungunya virus (CHIKV). CHIKV is expanding at an alarming rate, potentially spreading and establishing endemicity in new areas where competent vectors are present. The dramatic spread of CHIKV in recent years highlights the urgent need to take precautionary measures and investigate options for control. It is crucial in developing nations where diagnostic tools are limited, and symptoms are similar to other prevalent diseases such as malaria and dengue. The most reliable method for diagnosing chikungunya virus is viral gene detection by RT-PCR. Alternative methods like detecting human antibody and viral antigen can also be used, especially in areas where resources are limited. In this review, we summarize the limited data on antigen detection immunoassays. We further explain the essential structural elements of the virus to help comprehend the scientific concepts underlying the testing methods, as well as future methods and diagnostic approaches under investigation.
摘要：
基孔肯雅病是由基孔肯雅病毒（CHIKV）引起的蚊子传播的病毒性疾病。CHIKV正以惊人的速度扩张，在有能力的媒介存在的新地区可能传播和建立地方病。近年来，CHIKV的急剧传播凸显了迫切需要采取预防措施并研究控制方案。在诊断工具有限的发展中国家，这一点至关重要，症状与疟疾和登革热等其他流行疾病相似。诊断基孔肯雅病毒的最可靠方法是通过RT-PCR检测病毒基因。也可以使用替代方法，如检测人抗体和病毒抗原，特别是在资源有限的地区。在这次审查中,我们总结了抗原检测免疫测定的有限数据。我们进一步解释病毒的基本结构元素，以帮助理解测试方法背后的科学概念，以及正在调查的未来方法和诊断方法。
