Embase, PubMed, Web of Science, WANFANG DATA, VIP, and China National Knowledge Infrastructure (CNKI) were comprehensively searched until July 2022 for pertinent studies. The pooled standardized mean difference (SMD) with a 95% confidence interval (CI) was calculated by the fixed or random-effect model. In Mendelian randomization (MR) analysis on the causal relationship between serum DKK-1 level and AS risk, the inverse variance weighting method (IVW), MR-Egger regression, weighted median method, and weighted pattern method were applied. Sensitivity analyses, including the horizontal pleiotropy test, heterogeneity test, and leave-one-out test, were also performed.
The meta-analysis of 40 studies containing 2,371 AS patients and 1,633 healthy controls showed that there was no significant difference in DKK-1 serum level between AS patients and normal controls (pooled SMD=0.207, 95% CI =-0.418-0.832, P=0.516). The subgroup analysis of the CRP ≤ 10 mg/L group showed that AS patients had higher serum DKK-1 concentration than the healthy controls (SMD=2.267, 95% CI = 0.102-4.432, P=0.040). Similarly, MR analysis also demonstrated no significant association between DKK-1 serum level and AS (IVW OR=0.999, 95% CI = 0.989-1.008, P=0.800). All sensitivity analyses revealed consistent results.
There was no significant change in serum DKK-1 concentration between AS patients and healthy controls. In addition, no causal relationship exists between serum DKK-1 levels and AS risk.
Embase,PubMed,WebofScience,万方数据,VIP,和中国国家知识基础设施(CNKI)进行了全面搜索,直到2022年7月进行相关研究。通过固定或随机效应模型计算具有95%置信区间(CI)的合并标准化平均差(SMD)。在孟德尔随机化(MR)分析血清DKK-1水平与AS风险之间的因果关系,方差逆加权法(IVW),MR-Egger回归,加权中位数法,采用加权模式法。敏感性分析,包括水平多效性测试,异质性检验,和离开一次测试,也表演了。
对包含2,371例AS患者和1,633例健康对照者的40项研究的荟萃分析显示,AS患者与正常对照者血清DKK-1水平无显著差异(SMD=0.207,95%CI=-0.418-0.832,P=0.516)。CRP≤10mg/L组患者血清DKK-1浓度高于健康对照组(SMD=2.267,95%CI=0.102~4.432,P=0.040)。同样,MR分析还显示DKK-1血清水平与AS之间没有显着关联(IVWOR=0.999,95%CI=0.989-1.008,P=0.800)。所有敏感性分析都显示出一致的结果。
AS患者与健康对照组之间血清DKK-1浓度无明显变化。此外,血清DKK-1水平与AS风险之间无因果关系.