PDF(Pubmed)
Abstract：
Toxoplasma gondii is an apicomplexan parasite that affects the health of humans and livestock, and an effective vaccine is urgently required. Nanoparticles can modulate and improve cellular and humoral immune responses.
In the current study, poly (D, L-lactic-co-glycolic acid) (PLGA) nanoparticles were used as a delivery system for the T. gondii dense granule antigens GRA12 and GRA7. BALB/c mice were injected with the vaccines and protective efficacy was evaluated.
Mice immunized with PLGA+GRA12 exhibited significantly higher IgG, and a noticeable predominance of IgG2a over IgG1 was also observed. There was a 1.5-fold higher level of lymphocyte proliferation in PLGA+GRA12-injected mice compared to Alum+GRA12-immunized mice. Higher levels of IFN-g and IL-10 and a lower level of IL-4 were detected, indicating that Th1 and Th2 immune responses were induced but the predominant response was Th1. There were no significant differences between Alum+GRA7-immunized and PLGA+GRA7-immunized groups. Immunization with these four vaccines resulted in significantly reduced parasite loads, but they were lowest in PLGA+GRA12-immunized mice. The survival times of mice immunized with PLGA+GRA12 were also significantly longer than those of mice in the other vaccinated groups.
The current study indicated that T. gondii GRA12 recombinant protein encapsulated in PLGA nanoparticles is a promising vaccine against acute toxoplasmosis, but PLGA is almost useless for enhancing the immune response induced by T. gondii GRA7 recombinant protein.
In the current study, poly (D, L-lactic-co-glycolic acid) (PLGA) nanoparticles were used as a delivery system for the T. gondii dense granule antigens GRA12 and GRA7. BALB/c mice were injected with the vaccines and protective efficacy was evaluated.
Mice immunized with PLGA+GRA12 exhibited significantly higher IgG, and a noticeable predominance of IgG2a over IgG1 was also observed. There was a 1.5-fold higher level of lymphocyte proliferation in PLGA+GRA12-injected mice compared to Alum+GRA12-immunized mice. Higher levels of IFN-g and IL-10 and a lower level of IL-4 were detected, indicating that Th1 and Th2 immune responses were induced but the predominant response was Th1. There were no significant differences between Alum+GRA7-immunized and PLGA+GRA7-immunized groups. Immunization with these four vaccines resulted in significantly reduced parasite loads, but they were lowest in PLGA+GRA12-immunized mice. The survival times of mice immunized with PLGA+GRA12 were also significantly longer than those of mice in the other vaccinated groups.
The current study indicated that T. gondii GRA12 recombinant protein encapsulated in PLGA nanoparticles is a promising vaccine against acute toxoplasmosis, but PLGA is almost useless for enhancing the immune response induced by T. gondii GRA7 recombinant protein.
摘要：
弓形虫是一种影响人类和家畜健康的顶复虫寄生虫,迫切需要有效的疫苗。纳米颗粒可以调节和改善细胞和体液免疫应答。
在目前的研究中,聚(D,L-乳酸-共-乙醇酸)(PLGA)纳米颗粒用作弓形虫致密颗粒抗原GRA12和GRA7的递送系统。用疫苗注射BALB/c小鼠并评价保护效力。
用PLGA+GRA12免疫的小鼠表现出明显更高的IgG，并且还观察到IgG2a明显优于IgG1。与明矾+GRA12免疫的小鼠相比，注射PLGA+GRA12的小鼠淋巴细胞增殖水平高1.5倍。检测到较高水平的IFN-g和IL-10和较低水平的IL-4，表明诱导了Th1和Th2免疫反应，但主要反应是Th1。明矾+GRA7免疫组和PLGA+GRA7免疫组之间没有显著差异。用这四种疫苗免疫导致寄生虫负荷显著减少，但在PLGA+GRA12免疫小鼠中最低。用PLGA+GRA12免疫的小鼠的存活时间也显著长于其他接种组中的小鼠的存活时间。
目前的研究表明，包裹在PLGA纳米颗粒中的弓形虫GRA12重组蛋白是一种有前途的急性弓形虫病疫苗，但PLGA对增强弓形虫GRA7重组蛋白诱导的免疫反应几乎没有用。
在目前的研究中,聚(D,L-乳酸-共-乙醇酸)(PLGA)纳米颗粒用作弓形虫致密颗粒抗原GRA12和GRA7的递送系统。用疫苗注射BALB/c小鼠并评价保护效力。
用PLGA+GRA12免疫的小鼠表现出明显更高的IgG，并且还观察到IgG2a明显优于IgG1。与明矾+GRA12免疫的小鼠相比，注射PLGA+GRA12的小鼠淋巴细胞增殖水平高1.5倍。检测到较高水平的IFN-g和IL-10和较低水平的IL-4，表明诱导了Th1和Th2免疫反应，但主要反应是Th1。明矾+GRA7免疫组和PLGA+GRA7免疫组之间没有显著差异。用这四种疫苗免疫导致寄生虫负荷显著减少，但在PLGA+GRA12免疫小鼠中最低。用PLGA+GRA12免疫的小鼠的存活时间也显著长于其他接种组中的小鼠的存活时间。
目前的研究表明，包裹在PLGA纳米颗粒中的弓形虫GRA12重组蛋白是一种有前途的急性弓形虫病疫苗，但PLGA对增强弓形虫GRA7重组蛋白诱导的免疫反应几乎没有用。
