Abstract：
An automated accelerated salting-out assisted solvent extraction (A-ASASE) was developed. This approach made full use of the advantages of both the accelerated solvent extraction (ASE) technique and salting-out assisted liquid-liquid extraction (SALLE), without any modifications at the level of the ASE machine. The A-ASASE combined an automated extraction at high temperature/pressure and an enrichment step using eco-friendly solvents (e.g., water and ethanol) in a fully integrated and simple way that required only low volumes of solvents. The A-ASASE was successfully applied to extract three stilbenoids, namely E-resveratrol, E-ε-viniferin and E-vitisin B, present in grapevine (Vitis vinifera L.) by-products as a proof-of-concept compound. First, factors affecting the yield of stelbenoids by SALLE were investigated by means of a design of experiment model (DOE). The SALLE was then incorporated into ASE. Various ASE operational parameters such as temperature and number of cycles were also investigated. The highest yield was obtained using a mixture of ethanol-water, 70:30 (v/v) at 60 °C, using three extraction cycles and with 5 min of contact each. The highest yields of stilbenoids were 5.87 ± 0.28 g kg-1 dry weight, 3.27 ± 0.72 g kg-1 dry weight and 1.81 ± 0.2 g kg-1 dry weight for resveratrol, E-ϵ-viniferin and vitisin B, respectively. The A-ASASE was then successfully applied to extract the same stilbenoids from the roots of Vitis vinifera L. These results highlight the applicability of A-ASASE methodology to the extraction and enrichment of the extract in compounds of medium-high polarity in an easy and fully automated way.
摘要：
开发了一种自动加速盐析辅助溶剂萃取（A-ASASE）。这种方法充分利用了加速溶剂萃取（ASE）技术和盐析辅助液-液萃取（SALLE）技术的优点，无需在ASE机器级别进行任何修改。A-ASASE结合了高温/高压下的自动提取和使用环保溶剂的富集步骤（例如，水和乙醇）以完全集成和简单的方式，只需要少量的溶剂。A-ASASE已成功用于提取三种二苯乙烯类化合物，即E-白藜芦醇，E-ε-葡萄素和E-葡萄素B，作为概念验证化合物存在于葡萄（VitisviniferaL.）副产品中。首先,通过设计实验模型（DOE），研究了影响SALLE类星形化合物收率的因素。然后将SALLE掺入ASE中。还研究了各种ASE操作参数,例如温度和循环次数。使用乙醇-水的混合物获得最高的产率，70:30(v/v)在60°C,使用三个提取周期，每个接触5分钟。二苯乙烯的最高产量为5.87±0.28gkg-1干重，白藜芦醇的干重为3.27±0.72gkg-1，干重为1.81±0.2gkg-1，E-ε-葡萄素和葡萄素B，分别。然后将A-ASASE成功地应用于从葡萄L的根中提取相同的二苯乙烯类。这些结果突出了A-ASASE方法以简单和完全自动化的方式提取和富集中高极性化合物中的提取物的适用性。
