METHODS: In this study, a rat model of overuse injury was established by eccentric running for 4 weeks, and pressing at constant pressure and frequency and massage were used as intervention methods to explore whether massage could protect skeletal muscle from injury through upregulating integrin and the basement membrane laminin.
RESULTS: The results showed that compared with the model group, the ultrastructure of skeletal muscle in the massage group was relatively complete and clear, and the maximum isotonic and tetanic contraction forces were significantly increased (P < 0.01). In addition, in the massage group, β1 integrin expression was significantly increased, p-FAK protein expression was decreased, and the co-localization of β1 integrin and the basement membrane laminin 2 was significantly increased (P < 0.01).
CONCLUSIONS: Our study shows that during long-term heavy-duty exercise, massage can enhance the cell adhesion function mediated by integrin β1 and laminin 2 to protect skeletal muscle from injury and prevent the occurrence of overuse injury.
方法:在本研究中,通过偏心跑4周建立大鼠过度使用损伤模型,以恒定压力和频率按压和按摩为干预方法,探讨按摩是否可以通过上调整合素和基底膜层粘连蛋白来保护骨骼肌免受损伤。
结果:结果表明,与模型组相比,按摩组骨骼肌超微结构较为完整、清晰,最大等渗和强直收缩力显著增加(P<0.01)。此外,在按摩组,β1整合素表达显著增加,p-FAK蛋白表达降低,β1整合素与基底膜层粘连蛋白2的共定位显著增加(P<0.01)。
结论:我们的研究表明,在长期的重负荷运动中,按摩可以增强整合素β1和层粘连蛋白2介导的细胞粘附功能,保护骨骼肌免受损伤,防止过度使用损伤的发生。