PDF(Pubmed)
Abstract：
Dipylidium caninum infections in dogs and cats are underestimated because of a lack of proglottid observations and poor recovery of parasite elements by centrifugal flotation. We developed an immunoassay that employs a pair of monoclonal antibodies to capture D. caninum-specific coproantigen in fecal extracts from dogs and cats. Real-time PCR for D. caninum DNA in perianal swabs and observation of proglottids were used as reference methods. In 6 experimentally infected dogs, parasite DNA, coproantigen, and proglottid segments were first detected at 22, 23, and 26 d post-infection, respectively. Praziquantel treatment of 3 experimentally infected dogs resulted in the elimination of both coproantigen and proglottid shedding within 1-5 d post-treatment; however, parasite DNA persisted for 14 d. Immunohistochemistry on immature and mature tapeworm segments using an antibody against the coproantigen supports the premise that the antigen is produced in mature segments. We assessed the performance of our coproantigen test in natural infections in 78 dogs from a flea-endemic area. Of the 12 antigen-positive samples, 11 were confirmed with a positive PCR test and/or proglottid observation. Finally, we evaluated a convenience sample set of 730 canine and 163 feline fecal samples obtained from a commercial diagnostic laboratory; D. caninum antigen was detected in 4.1% of the canine and 12.9% of the feline samples, whereas parasite elements were observed in only 0.028% of samples. Our coproantigen immunoassay provides a sensitive method for the detection of D. caninum infection in dogs and cats.
摘要：
由于缺乏progloottid观察结果以及通过离心浮选对寄生虫元素的回收不良，因此低估了狗和猫的二吡喃杆菌感染。我们开发了一种免疫测定法，该免疫测定法采用一对单克隆抗体捕获狗和猫的粪便提取物中的D.caninum特异性共原抗原。肛周拭子中D.caninumDNA的实时PCR和前声门的观察被用作参考方法。在6只实验感染的狗中，寄生虫DNA,共原抗原，在感染后22、23和26d首次检测到poglotid节段，分别。吡喹酮对3只实验感染的狗的治疗导致治疗后1-5天内消除了前抗原和前声门蛋白的脱落；然而，寄生虫DNA持续14d。使用针对copro抗原的抗体对未成熟和成熟的tape虫片段进行免疫组织化学，支持抗原在成熟片段中产生的前提。我们评估了来自跳蚤流行地区的78只狗的自然感染中的共原抗原测试的性能。在12个抗原阳性样本中,通过PCR测试和/或progloottid观察结果证实了11例。最后,我们评估了从商业诊断实验室获得的730个犬科动物和163个猫科动物粪便样本的便利样本集;在4.1%的犬科动物和12.9%的猫科动物样本中检测到D.而寄生虫元素仅在0.028%的样品中观察到。我们的coproantigen免疫测定为检测狗和猫的D.caninum感染提供了一种灵敏的方法。
