Abstract：
LIF is crucial in regulating embryo implantation, while HOXA10 is a marker gene for uterine receptivity. However, the specific mechanism of LIF regulating HOXA10 during cow embryo implantation has not been fully understood. To address this knowledge gap, the experiment involved treating bovine endometrial epithelial cells (BEECs) with LIF to investigate the relationship between LIF, miRNA, and HOXA10. The experimental findings revealed that applying LIF resulted in a substantial increase in the proliferation of endometrial epithelial cells. Moreover, the expressions of PI3K, AKT, HOXA10, CDK4, cyclinD1, and cyclinE1 were significantly elevated. Conversely, the expression of p21Cipl was significantly reduced. In the group that received a combination of LIF and a STAT3 inhibitor, the expression of PI3K/AKT remained significantly increased, but there was no significant change in the expression of HOXA10. When miRNA-27a-3p was overexpressed, it resulted in a decrease in both the RNA and protein expression of HOXA10. Conversely, inhibiting miRNA-27a-3p increased the RNA and protein expression of HOXA10. In the presence of LIF treatment, the expression of miRNA-27a-3p was reduced, while the expression of HOXA10 was increased. However, when LIF and a STAT3 inhibitor were combined, there was no significant change in the expression of miRNA-27a-3p or HOXA10. Consequently, LIF facilitated cell proliferation by activating the PI3K/AKT pathway. LIF controlled the expression of miRNA-27a-3p and HOXA10 in endometrial epithelial cells through STAT3, with miRNA-27a-3p negatively regulating the expression of HOXA10.
摘要：
LIF在调节胚胎植入方面至关重要，而HOXA10是子宫容受性的标记基因。然而,LIF在奶牛胚胎着床过程中调节HOXA10的具体机制尚未完全了解。为了解决这个知识差距，本实验采用LIF处理牛子宫内膜上皮细胞(BEECs),miRNA,HOXA10实验结果表明，应用LIF可导致子宫内膜上皮细胞的增殖显着增加。此外，PI3K的表达式，AKT,HOXA10，CDK4，cyclinD1和cyclinE1显着升高。相反，p21Cipl的表达显著降低。在接受LIF和STAT3抑制剂组合的组中，PI3K/AKT的表达显著升高,但HOXA10的表达无显著改变。当miRNA-27a-3p过表达时，它导致HOXA10的RNA和蛋白质表达减少。相反，抑制miRNA-27a-3p增加HOXA10的RNA和蛋白质表达。在存在LIF治疗的情况下，miRNA-27a-3p的表达减少，而HOXA10的表达增加。然而,当LIF和STAT3抑制剂联合使用时，miRNA-27a-3p或HOXA10的表达无显著改变。因此,LIF通过激活PI3K/AKT途径促进细胞增殖。LIF通过STAT3控制子宫内膜上皮细胞miRNA-27a-3p和HOXA10的表达，miRNA-27a-3p负调控HOXA10的表达。
