METHODS: we have examined subjects with type 1 diabetes mellitus, vascular atherosclerotic disease, type 2 diabetes mellitus without and with macrovascular complications, chronic renal failure on conservative treatment, essential hypertension, deep venous thrombosis, acute ischemic stroke and subjects with venous leg ulcers.
METHODS: unfractioned leukocyte suspension was prepared according to the Mikita\'s method, while the leukocyte were separated into mononuclear and polymorphonuclear cells with a Ficoll-Hypaque medium. Using specific monoclonal antibody, the CD18 expression was evaluated with cytofluorimetric analysis, using FACScan (Becton Dickinson) be Cellquest software; the activation in vitro with PMA was effected according to modified Yasui and Masuda methods.
RESULTS: in type 1 diabetes mellitus, at baseline CD18 is under expressed in comparison with normal control, and not changes after PMA activation were observed; in subjects with vascular atherosclerotic disease, in type 2 diabetes mellitus CD18 is over expressed at baseline but does not vary after activation; in subjects with chronic renal failure, essential hypertension and in subjects with acute ischemic stroke the CD18 up-regulate at baseline compared to normal control, and it increases further after activation; in subjects with deep venous thrombosis the CD18 expression is not different from control group at baseline, but it increases after activation; finally, in subjects with venous leg ulcers the CD18 is normally expressed at baseline, and it does not change after PMA activation.
CONCLUSIONS: in the different clinical disorders, the trend of this integrin subunit provides some specific information, useful to select the best therapeutic strategy in clinical practice.
方法:我们检查了1型糖尿病患者,血管动脉粥样硬化疾病,无大血管并发症的2型糖尿病,慢性肾功能衰竭保守治疗,原发性高血压,深静脉血栓形成,急性缺血性中风和下肢静脉性溃疡患者。
方法:根据Mikita方法制备未分级的白细胞悬液,而白细胞用Ficoll-Hypaque培养基分离为单核细胞和多形核细胞。使用特异性单克隆抗体,用细胞荧光分析评估CD18的表达,使用FACScan(BectonDickinson)是Cellquest软件;根据改良的Yasui和Masuda方法进行PMA的体外激活。
结果:在1型糖尿病中,与正常对照相比,基线CD18表达不足,并且在PMA激活后没有观察到变化;在患有血管动脉粥样硬化疾病的受试者中,在2型糖尿病中,CD18在基线时过表达,但在激活后没有变化;在患有慢性肾功能衰竭的受试者中,与正常对照相比,原发性高血压和急性缺血性卒中患者的CD18在基线时上调,激活后进一步增加;在深静脉血栓形成的受试者中,基线时CD18的表达与对照组没有差异,但它在激活后增加;最后,在静脉性腿部溃疡的受试者中,CD18通常在基线表达,PMA激活后不会改变。
结论:在不同的临床疾病中,这个整合素亚基的趋势提供了一些特定的信息,有助于在临床实践中选择最佳的治疗策略。