Abstract：
Taf14 is a subunit of multiple fundamental complexes implicated in transcriptional regulation and DNA damage repair in yeast cells. Here, we investigate the association of Taf14 with the consensus sequence present in other subunits of these complexes and describe the mechanistic features that affect this association. We demonstrate that the precise molecular mechanisms and biological outcomes underlying the Taf14 interactions depend on the accessibility of binding interfaces, the ability to recognize other ligands, and a degree of sensitivity to temperature and chemical and osmotic stresses. Our findings aid in a better understanding of how the distribution of Taf14 among the complexes is mediated.
摘要：
Taf14是酵母细胞中涉及转录调控和DNA损伤修复的多个基本复合物的亚基。这里,我们研究了Taf14与这些复合物其他亚基中存在的共有序列的关联,并描述了影响这种关联的机制特征.我们证明了Taf14相互作用的精确分子机制和生物学结果取决于结合界面的可及性，识别其他配体的能力，以及对温度、化学和渗透应力的敏感性。我们的发现有助于更好地理解Taf14在复合物中的分布是如何介导的。
