Abstract：
The lateral hypothalamus\' orexinergic system has been associated with anxiety-related behaviors, and electroacupuncture (EA) modifies orexin neurons to control the anti-anxiety process. However, in a rat model of post-traumatic stress disorder (PTSD), the important role of LH orexin neurons (OXNs) in the anxiolytic effects induced by EA has not been explored. In this study, rats underwent modified single prolonged stress (MSPS) for seven days before developing EA. The rats were then subjected to elevated plus maze (EPM) and open field (OFT) tests, and western blot and c-Fos/orexin double labeling investigations were carried out to determine the functional activation of LH orexinergic neurons. Compared to MSPS model rats, it has been demonstrated that EA stimulation enhanced the amount of time spent in the central zone (TSCZ) in OFT and the amount of time spent in the open arm (TSOA) in EPM in MSPS model rats (P < 0.01). After behavioral testing, MSPS model rats had decreased activated c-Fos positive OXNs. Still, EA in SPS rats increased that number and elevated orexin type 1 receptors (OXR1) protein expression in the LH. Furthermore, after administering SB334867 (an OXR1 antagonist) to MSPS model rats, the effects of EA therapy on anxiety-like behaviors (ALBs) were significantly diminished. Additionally, when low-dose orexin-A (LORXA) was administered intracerebroventricularly together with EA stimulation in MSPS rats, the anxiolytic effects of the stimulation were substantially enhanced (P < 0.05). The results of this study reveal the mechanisms by which acupuncture may reduce PTSD and advance our understanding of the function of LH orexin signaling in EA\'s anxiolytic effects.
摘要：
下丘脑外侧氧化系统与焦虑相关行为有关，电针（EA）修饰食欲素神经元以控制抗焦虑过程。然而,在创伤后应激障碍（PTSD）的大鼠模型中，LH食欲素神经元（OXN）在EA诱导的抗焦虑作用中的重要作用尚未被研究。在这项研究中,在发生EA之前，大鼠接受了改良的单次延长应激（MSPS）7天。然后对大鼠进行高架迷宫（EPM）和开放场（OFT）测试，进行了蛋白质印迹和c-Fos/食欲素双重标记研究，以确定LH食欲能神经元的功能激活。与MSPS模型大鼠相比，已经证明，EA刺激增加了MSPS模型大鼠OFT中在中心区（TSCZ）花费的时间和在EPM中在开放臂（TSOA）花费的时间（P<0.01）。经过行为测试，MSPS模型大鼠的活化c-Fos阳性OXNs降低。尽管如此，SPS大鼠的EA增加了LH中食欲素1型受体（OXR1）的数量和升高的蛋白表达。此外，向MSPS模型大鼠施用SB334867（一种OXR1拮抗剂）后，EA治疗对焦虑样行为(ALB)的影响显著减弱.此外,当低剂量食欲素A（LORXA）与EA刺激一起在MSPS大鼠脑室内给药，刺激的抗焦虑作用显着增强（P<0.05）。这项研究的结果揭示了针灸可能减少PTSD的机制，并增进了我们对LH食欲素信号在EA抗焦虑作用中的作用的理解。
