PDF(Pubmed)
Abstract：
The high-throughput sequencing at single-cell resolution requires high-quality samples of inputted 100-10,000 cells with at least 80%-90% viability according to the applied platform. Here, we present a protocol for single-cell isolation from normal and neoplastic human pancreas. We describe steps for sample harvesting, procurement, tissue digestion, and cell purification. Subsequently, isolated cells can be further applied to single-cell profiling, for example, single-cell RNA sequencing and single-cell ATAC-seq using 10× Genomics platform. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019)1 and Li et al. (2021).2.
摘要：
在单细胞分辨率下的高通量测序需要输入的100-10,000个细胞的高质量样品,根据应用的平台具有至少80%-90%的活力。这里,我们提出了从正常和肿瘤人类胰腺中分离单细胞的方案。我们描述了样品采集的步骤，采购,组织消化，和细胞纯化。随后，分离的细胞可以进一步应用于单细胞谱分析，例如,使用10×基因组学平台进行单细胞RNA测序和单细胞ATAC-seq。有关此协议的使用和执行的完整详细信息，请参考彭等人。（2019）1和李等人。（2021年）.2。
