PDF(Pubmed)
Abstract：
Wound healing in DFU (diabetic foot ulcer) has prolonged inflammation phase and defective granulation tissue formation. LL-37 has antimicrobial property, induces angiogenesis, and keratinocyte migration and proliferation. This study analyzes the efficacy of LL-37 cream in enhancing wound healing rate and decreasing the levels of IL-1α, TNF-α, and the number of aerobic bacteria colonization in DFU with mild infection. This study was conducted from January 2020 to June 2021 in Jakarta. Subjects were instructed to apply either LL-37 cream or placebo cream twice a week for 4 weeks. Wounds were measured on days 7, 14, 21, and 28 and processed with ImageJ. The levels of LL-37, IL-1α, and TNF-α from wound fluid were measured using ELISA. The number of aerobic bacteria colonization was counted from the isolate grown in culture. The levels of LL-37 in DFU at baseline were equally low in both groups which were 1.07 (0.37-4.96) ng/mg protein in the LL-37 group and 1.11 (0.24-2.09) ng/mg protein in the placebo group. The increase in granulation index was consistently greater in the LL-37 group on days 7, 14, 21, and 28 (p = 0.031, 0.009, 0.006, and 0.037, respectively). The levels of IL-1α and TNF-α increased in both groups on days 14 and 21 (p > 0.05). The decrease in the number of aerobic bacteria colonization was greater in the LL-37 group on days 7, 14 and 21, but greater in the placebo group on day 28 (p > 0.05). In conclusion, LL-37 cream enhanced the healing rate of DFU with mild infection, but did not decrease the levels of IL-1α and TNF-α and the number of aerobic bacteria colonization. This trial is registered at ClinicalTrials.gov, number NCT04098562.
摘要：
DFU（糖尿病足溃疡）中的伤口愈合具有延长的炎症阶段和有缺陷的肉芽组织形成。LL-37具有抗菌性能,诱导血管生成，和角质形成细胞迁移和增殖。本研究分析了LL-37乳膏在提高伤口愈合率和降低IL-1α水平方面的功效。TNF-α,以及在轻度感染的DFU中需氧菌定植的数量。这项研究于2020年1月至2021年6月在雅加达进行。指示受试者每周两次施用LL-37乳膏或安慰剂乳膏,持续4周。在第7、14、21和28天测量创伤并用ImageJ处理。LL-37、IL-1α、使用ELISA测量伤口液中的TNF-α。从培养物中生长的分离物计数需氧菌定殖的数量。基线时DFU中LL-37的水平在两组中同样低,在LL-37组中为1.07(0.37-4.96)ng/mg蛋白,在安慰剂组中为1.11(0.24-2.09)ng/mg蛋白。在第7、14、21和28天，LL-37组的颗粒指数增加始终更大（分别为p=0.031、0.009、0.006和0.037）。在第14天和第21天，两组的IL-1α和TNF-α水平均升高（p>0.05）。需氧细菌定植数量的减少在LL-37组中在第7、14和21天更大,但在安慰剂组中在第28天更大(p>0.05)。总之,LL-37乳膏可提高DFU轻度感染的治愈率，但没有降低IL-1α和TNF-α的水平和需氧菌定植的数量。该试验在ClinicalTrials.gov注册，编号NCT04098562。
