PDF(Pubmed)
Abstract：
BACKGROUND: Pericardial effusions are one of the most common cardiac diseases in dogs. Common causes of haemorrhagic pericardial effusions include neoplasia, such as hemangiosarcoma, mesothelioma, chemodectoma, and ectopic thyroid tumours, and benign idiopathic pericardial effusion. Distinguishing among reactive mesothelial cells, malignant mesothelioma, and adenocarcinoma in body effusions is a diagnostic challenge. Therefore, the author aimed to discover whether the observed cells were reactive mesothelial, mesothelioma, or adenocarcinoma cells through immunocytochemistry using five markers (cytokeratin, vimentin, desmin, E-cadherin, and calretinin) in a canine patient.
METHODS: A 2.1 kg, spayed female, 10-year-old Yorkshire Terrier dog presented to a local hospital with dyspnoea and was evaluated for pericardial effusion. The presence of pericardial fluid was confirmed, and she was referred to our hospital for further evaluation. In cytological evaluation, cells shed individually or in clusters were observed, along with numerous non-degenerative neutrophils and macrophages. The cells showed binucleation, anisocytosis, anisokaryosis, abnormal nucleoli, abundant basophilic cytoplasm, high nuclear-cytoplasmic ratio, and coarse chromatin. Large atypical multinucleate cells were also observed. Erythrophagia was observed, indicating chronic haemorrhage. Immunocytochemistry using pericardial fluid was positive for cytokeratin, vimentin, desmin, E-cadherin, and calretinin. Therefore, malignant mesothelioma was diagnosed.
CONCLUSIONS: Immunocytochemistry is a very useful diagnostic technique because it can determine whether several fluorescent markers are simultaneously expressed in the same cell. Further, E-cadherin and calretinin can be used for the differential diagnosis of reactive mesothelial cells, malignant mesothelioma, and adenocarcinoma in dogs.
METHODS: A 2.1 kg, spayed female, 10-year-old Yorkshire Terrier dog presented to a local hospital with dyspnoea and was evaluated for pericardial effusion. The presence of pericardial fluid was confirmed, and she was referred to our hospital for further evaluation. In cytological evaluation, cells shed individually or in clusters were observed, along with numerous non-degenerative neutrophils and macrophages. The cells showed binucleation, anisocytosis, anisokaryosis, abnormal nucleoli, abundant basophilic cytoplasm, high nuclear-cytoplasmic ratio, and coarse chromatin. Large atypical multinucleate cells were also observed. Erythrophagia was observed, indicating chronic haemorrhage. Immunocytochemistry using pericardial fluid was positive for cytokeratin, vimentin, desmin, E-cadherin, and calretinin. Therefore, malignant mesothelioma was diagnosed.
CONCLUSIONS: Immunocytochemistry is a very useful diagnostic technique because it can determine whether several fluorescent markers are simultaneously expressed in the same cell. Further, E-cadherin and calretinin can be used for the differential diagnosis of reactive mesothelial cells, malignant mesothelioma, and adenocarcinoma in dogs.
摘要：
背景：心包积液是狗最常见的心脏疾病之一。出血性心包积液的常见原因包括瘤形成，比如血管肉瘤，间皮瘤,化学切除瘤,和异位甲状腺肿瘤，良性特发性心包积液.区分反应性间皮细胞，恶性间皮瘤,体内积液中的腺癌是一个诊断挑战。因此,作者旨在发现观察到的细胞是否为反应性间皮，间皮瘤,或腺癌细胞通过免疫细胞化学使用五个标记(细胞角蛋白，波形蛋白,desmin,E-cadherin,和calretinin）在犬科患者中。
方法：A2.1公斤，Spyed女性，10岁的约克郡梗犬因呼吸困难被送往当地医院，并进行了心包积液评估。证实存在心包液,她被转诊到我们医院接受进一步评估。在细胞学评估中，观察到单独或成簇脱落的细胞，以及许多非变性的中性粒细胞和巨噬细胞。细胞显示出双核，异细胞增多症,anisokaryosis,异常的核仁,丰富的嗜碱性细胞质，高核质比，和粗染色质。还观察到大的非典型多核细胞。观察到红食症，表明慢性出血。使用心包液的免疫细胞化学对细胞角蛋白呈阳性，波形蛋白,desmin,E-cadherin,还有卡列丁.因此,诊断为恶性间皮瘤。
结论：免疫细胞化学是一种非常有用的诊断技术，因为它可以确定几种荧光标记物是否在同一细胞中同时表达。Further,E-cadherin和calretinin可用于反应性间皮细胞的鉴别诊断，恶性间皮瘤,和狗的腺癌。
方法：A2.1公斤，Spyed女性，10岁的约克郡梗犬因呼吸困难被送往当地医院，并进行了心包积液评估。证实存在心包液,她被转诊到我们医院接受进一步评估。在细胞学评估中，观察到单独或成簇脱落的细胞，以及许多非变性的中性粒细胞和巨噬细胞。细胞显示出双核，异细胞增多症,anisokaryosis,异常的核仁,丰富的嗜碱性细胞质，高核质比，和粗染色质。还观察到大的非典型多核细胞。观察到红食症，表明慢性出血。使用心包液的免疫细胞化学对细胞角蛋白呈阳性，波形蛋白,desmin,E-cadherin,还有卡列丁.因此,诊断为恶性间皮瘤。
结论：免疫细胞化学是一种非常有用的诊断技术，因为它可以确定几种荧光标记物是否在同一细胞中同时表达。Further,E-cadherin和calretinin可用于反应性间皮细胞的鉴别诊断，恶性间皮瘤,和狗的腺癌。
