PDF(Pubmed)
Abstract：
The ESOT TLJ 3.0. consensus conference brought together leading experts in transplantation to develop evidence-based guidance on the standardization and clinical utility of pre-implantation kidney biopsy in the assessment of grafts from Expanded Criteria Donors (ECD). Seven themes were selected and underwent in-depth analysis after formulation of PICO (patient/population, intervention, comparison, outcomes) questions. After literature search, the statements for each key question were produced, rated according the GRADE approach [Quality of evidence: High (A), Moderate (B), Low (C); Strength of Recommendation: Strong (1), Weak (2)]. The statements were subsequently presented in-person at the Prague kick-off meeting, discussed and voted. After two rounds of discussion and voting, all 7 statements reached an overall agreement of 100% on the following issues: needle core/wedge/punch technique representatively [B,1], frozen/paraffin embedded section reliability [B,2], experienced/non-experienced on-call renal pathologist reproducibility/accuracy of the histological report [A,1], glomerulosclerosis/other parameters reproducibility [C,2], digital pathology/light microscopy in the measurement of histological variables [A,1], special stainings/Haematoxylin and Eosin alone comparison [A,1], glomerulosclerosis reliability versus other histological parameters to predict the graft survival, graft function, primary non-function [B,1]. This methodology has allowed to reach a full consensus among European experts on important technical topics regarding pre-implantation biopsy in the ECD graft assessment.
摘要：
ESOTTLJ3.0。共识会议汇集了移植方面的领先专家,就移植前肾活检在评估扩大标准供体(ECD)移植物方面的标准化和临床实用性制定循证指导.选择了七个主题，并在制定PICO后进行了深入分析(患者/人群，干预，比较,结果)问题。经过文献检索,产生了每个关键问题的陈述，根据等级方法[证据质量：高(A)，中度（B），低(C)；推荐强度：强(1)，弱（2）]。声明随后在布拉格启动会议上亲自介绍，讨论和投票。经过两轮讨论和表决,所有7项声明在以下问题上达成了100％的总体共识：代表性的针芯/楔形/打孔技术[B，1],冷冻/石蜡包埋切片可靠性[B，2],有经验/无经验的随叫随到的肾脏病理学家的组织学报告的可重复性/准确性[A，1],肾小球硬化/其他参数可重复性[C，2],数字病理学/光学显微镜在组织学变量测量中的应用[A，1],特殊染色/苏木精和伊红单独比较[A，1],肾小球硬化可靠性与其他组织学参数，以预测移植物存活，移植物功能，主要的非功能[B，1].这种方法使欧洲专家在ECD移植物评估中关于植入前活检的重要技术主题上达成了完全共识。
