METHODS: The purpose of this study was to analyze in silico the differentially expressed genes from five microarray datasets of patients with CRC. Furthermore, miRNAs were investigated to inhibit cancer cell proliferation and metastasis by targeting a key gene-frizzled receptor 3 (FZD3) in the Wnt signaling pathway.
RESULTS: The Wnt pathway receptor FZD3 is upregulated in CRC along with other pathway genes, which play a critical role in tumorigenesis. In contrast, miR-98-5p inhibits the activity of FZD3 by binding directly to the 3\'UTR of its mRNA, therefore exerting a suppressor effect on colorectal tumors.
CONCLUSIONS: The study reveals miR-98-5p as a novel target of FZD3 and an inhibitor of the Wnt signaling pathway hence being a potential candidate for developing targeted therapies against CRC.
方法:本研究的目的是分析来自5个CRC患者微阵列数据集的差异表达基因。此外,研究了通过靶向Wnt信号通路中的关键基因卷曲受体3(FZD3)来抑制癌细胞增殖和转移的miRNA。
结果:在CRC中Wnt通路受体FZD3与其他通路基因一起上调,在肿瘤发生中起关键作用。相比之下,miR-98-5p通过直接结合其mRNA的3'UTR抑制FZD3的活性,因此对结肠直肠肿瘤有抑制作用。
结论:该研究揭示miR-98-5p作为FZD3的新靶标和Wnt信号通路的抑制剂,因此是开发针对CRC的靶向治疗的潜在候选者。