Abstract：
The mammalian reproductive cycle, including those of humans and mice, begins very early in development. In utero, the ovaries become populated with primordial germ cells (PGCs) that will generate the oogonia. First, these cells proliferate mitotically, and then they trigger the meiotic program and initiate meiotic prophase I. Since these processes happen during gestation, their study had been very limited and challenging. Recently, we reported that, in the naked mole-rat (Heterocephalus glaber) ovary, there is mitotic expansion of the PGCs, and the initiation of the meiotic program occurs postnatally. In this chapter, we present a comprehensive collection of protocols that permit the analysis of naked mole-rat germ cells, from PGCs to oocytes, in meiotic prophase I, using in vivo and in vitro approaches.
摘要：
哺乳动物的生殖周期,包括人类和老鼠，在发展的早期就开始了。在子宫内,卵巢充满了原始生殖细胞（PGCs），将产生卵原。首先,这些细胞有丝分裂地增殖，然后他们触发减数分裂程序并启动减数分裂前期I。由于这些过程发生在妊娠期间，他们的研究非常有限和具有挑战性。最近,我们报告说，在裸痣大鼠（异头鼠）卵巢中，PGCs有丝分裂扩张,减数分裂程序的开始发生在出生后。在这一章中,我们提出了一个全面的方案，允许分析裸痣大鼠生殖细胞，从PGCs到卵母细胞，在减数分裂前期I,使用体内和体外方法。
