PDF(Pubmed)
Abstract：
Insulin signaling plays a critical role in regulating various aspects of insect biology, including development, reproduction, and the formation of wing polyphenism. This leads to differentiation among insect populations at different levels. The insulin family exhibits functional variation, resulting in diverse functional pathways. Aphis gossypii Glover, commonly known as the cotton-melon aphid, is a highly adaptable aphid species that has evolved into multiple biotypes. To understand the genetic structure of the insulin family and its evolutionary diversification and expression patterns in A. gossypii, we conducted studies using genome annotation files and RNA-sequencing data. Consequently, we identified 11 insulin receptor protein (IRP) genes in the genomes of the examined biotypes. Among these, eight AgosIRPs were dispersed across the X chromosome, while two were found in tandem on the A1 chromosome. Notably, AgosIRP2 exhibited alternative splicing, resulting in the formation of two isoforms. The AgosIRP genes displayed a high degree of conservation between Hap1 and Hap3, although some variations were observed between their genomes. For instance, a transposon was present in the coding regions of AgosIRP3 and AgosIRP9 in the Hap3 genome but not in the Hap1 genome. RNA-sequencing data revealed that four AgosIRPs were expressed ubiquitously across different morphs of A. gossypii, while others showed specific expression patterns in adult gynopara and adult males. Furthermore, the expression levels of most AgosIRPs decreased upon treatment with the pesticide acetamiprid. These findings demonstrate the evolutionary diversification of AgosIRPs between the genomes of the two biotypes and provide insights into their expression profiles across different morphs, developmental stages, and biotypes. Overall, this study contributes valuable information for investigating aphid genome evolution and the functions of insulin receptor proteins.
摘要：
胰岛素信号在调节昆虫生物学的各个方面中起着至关重要的作用。包括发展,繁殖，以及机翼多表型的形成。这导致不同水平的昆虫种群之间的差异。胰岛素家族表现出功能变异，导致不同的功能途径。AphisgossypiiGlover,通常被称为棉瓜蚜虫，是一种适应性很强的蚜虫物种，已经进化成多种生物型。了解胰岛素家族的遗传结构及其在棉草中的进化多样性和表达模式，我们使用基因组注释文件和RNA测序数据进行了研究.因此,我们在检测的生物型基因组中鉴定出11个胰岛素受体蛋白(IRP)基因.其中,八个AgosIRPs分散在X染色体上，而在A1染色体上发现了两个串联。值得注意的是,AgosIRP2表现出选择性剪接，导致两种同工型的形成。AgosIRP基因在Hap1和Hap3之间显示出高度的保守性，尽管在它们的基因组之间观察到一些变异。例如,转座子存在于Hap3基因组中AgosIRP3和AgosIRP9的编码区，但不存在于Hap1基因组中。RNA测序数据显示，四个AgosIRP在不同形态的A.gossypii中普遍表达，而其他人则在成年gynopara和成年男性中显示出特定的表达模式。此外，用杀虫剂啶虫脒处理后，大多数AgosIRP的表达水平降低。这些发现证明了AgosIRP在两种生物型基因组之间的进化多样化，并提供了对它们在不同形态中的表达谱的见解。发育阶段,和生物型。总的来说,这项研究为研究蚜虫基因组进化和胰岛素受体蛋白的功能提供了有价值的信息。
