Abstract：
For the digestion of food, it is important for the gut to be differentiated regionally and to have proper motor control. However, the number of transcription factors that regulate its development is still limited. Meanwhile, the interstitial cells of the gastrointestinal (GI) tract are necessary for intestinal motility in addition to the enteric nervous system. There are anoctamine1 (Ano1)-positive and platelet-derived growth factor receptor α (Pdgfra)-positive interstitial cells in mammal, but Pdgfra-positive cells have not been reported in the zebrafish. To identify new transcription factors involved in GI tract development, we used RNA sequencing comparing between larval and adult gut. We isolated 40 transcription factors that were more highly expressed in the larval gut. We demonstrated expression patterns of the 13 genes, 7 of which were newly found to be expressed in the zebrafish larval gut. Six of the 13 genes encode nuclear receptors. The osr2 is expressed in the anterior part, while foxP4 in its distal part. Also, we reported the expression pattern of pdgfra for the first time in the larval zebrafish gut. Our data provide fundamental knowledge for studying vertebrate gut regionalization and motility by live imaging using zebrafish.
摘要：
为了消化食物，重要的是要区分肠道的区域和有适当的运动控制。然而,调节其发育的转录因子的数量仍然有限。同时,除了肠神经系统之外,胃肠(GI)道的间质细胞对于肠运动是必需的。哺乳动物中存在Anoctamine1（Ano1）阳性和血小板衍生生长因子受体α（Pdgfra）阳性的间质细胞，但是斑马鱼中没有Pdgfra阳性细胞的报道。为了鉴定参与胃肠道发育的新转录因子，我们使用RNA测序对幼虫和成年肠道进行比较。我们分离了40个在幼虫肠道中表达更高的转录因子。我们展示了13个基因的表达模式，其中7个新发现在斑马鱼幼虫肠道中表达。13个基因中的6个编码核受体。osr2在前部表达，而foxP4，在它的远端。此外，我们首次报道了pdgfra在斑马鱼幼虫肠道中的表达模式。我们的数据将为使用斑马鱼通过实时成像研究脊椎动物肠道区域化和运动性提供基础知识。本文受版权保护。保留所有权利。
