UNASSIGNED: Ten ATLL and 10 healthy control individuals were investigated in this study. We used ELISA test to screen anti-HTLV-I antibodies and PCR for confirmation of infection. Then, we extracted total RNA from fresh whole blood, and cDNA was synthesized. The expression levels of the MAP2K1 gene were examined by qRT-PCR, and to check possible mutations in the MAP2K1 gene; all samples were sequenced and analyzed by BioEdite Software.
UNASSIGNED: MAP2K1 gene expression in the ATLL group was significantly higher than in the healthy control (P=0.001). The mutational sequencing analysis showed nucleotide 212 (S→R) change and identification mutations at different nucleotides that were entirely different from the nucleotide mutations defined in the UniProt database.
UNASSIGNED: These results could be a perspective in the prevention, prognosis, and targeted treatment of diseases in which the MAP2K1 gene plays a vital role.
■本研究调查了10名ATLL和10名健康对照个体。我们使用ELISA测试来筛选抗HTLV-I抗体,并使用PCR来确认感染。然后,我们从新鲜的全血中提取总RNA,并合成了cDNA。通过qRT-PCR检测MAP2K1基因的表达水平,并检查MAP2K1基因中可能的突变;所有样品均通过BioEdite软件进行测序和分析。
■ATLL组MAP2K1基因表达显著高于健康对照组(P=0.001)。突变测序分析显示核苷酸212(S→R)变化和不同核苷酸处的鉴定突变,其与UniProt数据库中定义的核苷酸突变完全不同。
■这些结果可能是预防的视角,预后,MAP2K1基因在疾病的靶向治疗中起着至关重要的作用。