PDF(Pubmed)
Abstract：
UNASSIGNED: HTLV-1 is responsible for two important diseases, HAM/TSP and ATLL. Approximately 10 to 20 million people are infected with HTLV-1 worldwide. Identifying altered genes in different cancers is crucial for finding potential treatment strategies. One of the proteins of the RAS/MAPK signaling pathway is MEK1, which is made from the MAP2K1 gene. The effects of the MAP2K1 gene on the MAPK signaling pathway are not yet fully elucidated. The current study aims to determine the MAP2K1 gene mutations and the level of MAP2K1 gene expression in ATLL patients compared to healthy individuals.
UNASSIGNED: Ten ATLL and 10 healthy control individuals were investigated in this study. We used ELISA test to screen anti-HTLV-I antibodies and PCR for confirmation of infection. Then, we extracted total RNA from fresh whole blood, and cDNA was synthesized. The expression levels of the MAP2K1 gene were examined by qRT-PCR, and to check possible mutations in the MAP2K1 gene; all samples were sequenced and analyzed by BioEdite Software.
UNASSIGNED: MAP2K1 gene expression in the ATLL group was significantly higher than in the healthy control (P=0.001). The mutational sequencing analysis showed nucleotide 212 (S→R) change and identification mutations at different nucleotides that were entirely different from the nucleotide mutations defined in the UniProt database.
UNASSIGNED: These results could be a perspective in the prevention, prognosis, and targeted treatment of diseases in which the MAP2K1 gene plays a vital role.
UNASSIGNED: Ten ATLL and 10 healthy control individuals were investigated in this study. We used ELISA test to screen anti-HTLV-I antibodies and PCR for confirmation of infection. Then, we extracted total RNA from fresh whole blood, and cDNA was synthesized. The expression levels of the MAP2K1 gene were examined by qRT-PCR, and to check possible mutations in the MAP2K1 gene; all samples were sequenced and analyzed by BioEdite Software.
UNASSIGNED: MAP2K1 gene expression in the ATLL group was significantly higher than in the healthy control (P=0.001). The mutational sequencing analysis showed nucleotide 212 (S→R) change and identification mutations at different nucleotides that were entirely different from the nucleotide mutations defined in the UniProt database.
UNASSIGNED: These results could be a perspective in the prevention, prognosis, and targeted treatment of diseases in which the MAP2K1 gene plays a vital role.
摘要：
■HTLV-1是两种重要的疾病，HAM/TSP和ATLL。全世界大约有1000万到2000万人感染了HTLV-1。识别不同癌症中改变的基因对于找到潜在的治疗策略至关重要。RAS/MAPK信号通路的蛋白之一是MEK1,其由MAP2K1基因制成。MAP2K1基因对MAPK信号通路的影响尚未完全阐明。本研究旨在确定与健康个体相比，ATLL患者的MAP2K1基因突变和MAP2K1基因表达水平。
■本研究调查了10名ATLL和10名健康对照个体。我们使用ELISA测试来筛选抗HTLV-I抗体，并使用PCR来确认感染。然后，我们从新鲜的全血中提取总RNA,并合成了cDNA。通过qRT-PCR检测MAP2K1基因的表达水平，并检查MAP2K1基因中可能的突变；所有样品均通过BioEdite软件进行测序和分析。
■ATLL组MAP2K1基因表达显著高于健康对照组(P=0.001)。突变测序分析显示核苷酸212(S→R)变化和不同核苷酸处的鉴定突变,其与UniProt数据库中定义的核苷酸突变完全不同。
■这些结果可能是预防的视角，预后,MAP2K1基因在疾病的靶向治疗中起着至关重要的作用。
■本研究调查了10名ATLL和10名健康对照个体。我们使用ELISA测试来筛选抗HTLV-I抗体，并使用PCR来确认感染。然后，我们从新鲜的全血中提取总RNA,并合成了cDNA。通过qRT-PCR检测MAP2K1基因的表达水平，并检查MAP2K1基因中可能的突变；所有样品均通过BioEdite软件进行测序和分析。
■ATLL组MAP2K1基因表达显著高于健康对照组(P=0.001)。突变测序分析显示核苷酸212(S→R)变化和不同核苷酸处的鉴定突变,其与UniProt数据库中定义的核苷酸突变完全不同。
■这些结果可能是预防的视角，预后,MAP2K1基因在疾病的靶向治疗中起着至关重要的作用。
