Abstract：
Exosomes derived from stem cells are a potential cell-free tool for tissue regeneration with therapeutic potential. However, its application in cementum repair is unclear. This study aimed to investigate the effect of human periodontal ligament stem cell-derived exosomes on the biological activity of cementoblasts, the main effector cells in cementum synthesis.
OCCM-30 cementoblasts were cultured with various human periodontal ligament stem cell-derived exosome concentrations. OCCM-30 cells proliferation, migration, and cementogenic mineralization were examined, along with the gene and protein expression of factors associated with cementoblastic mineralization.
Exosomal promoted the migration, proliferation, and mineralization of OCCM-30 cells. The exosome-treated group significantly increased the expression of cementogenic-related genes and proteins. Furthermore, the expression of p-PI3K and p-AKT was enhanced by exosome administration. Treatment with a PI3K/AKT inhibitor markedly attenuated the gene and protein expression of cementoblastic factors, and this effect was partially reversed by exosome administration.
Human periodontal ligament stem cell-derived exosomes can promote the activity of cementoblasts via the PI3K/AKT signaling pathway, providing a scientific basis for promoting the repair process in orthodontically induced inflammatory root resorption.
OCCM-30 cementoblasts were cultured with various human periodontal ligament stem cell-derived exosome concentrations. OCCM-30 cells proliferation, migration, and cementogenic mineralization were examined, along with the gene and protein expression of factors associated with cementoblastic mineralization.
Exosomal promoted the migration, proliferation, and mineralization of OCCM-30 cells. The exosome-treated group significantly increased the expression of cementogenic-related genes and proteins. Furthermore, the expression of p-PI3K and p-AKT was enhanced by exosome administration. Treatment with a PI3K/AKT inhibitor markedly attenuated the gene and protein expression of cementoblastic factors, and this effect was partially reversed by exosome administration.
Human periodontal ligament stem cell-derived exosomes can promote the activity of cementoblasts via the PI3K/AKT signaling pathway, providing a scientific basis for promoting the repair process in orthodontically induced inflammatory root resorption.
摘要：
目的：干细胞来源的外泌体是一种潜在的无细胞组织再生工具，具有治疗潜力。然而,其在牙骨质修复中的应用尚不清楚。本研究旨在探讨人牙周膜干细胞来源的外泌体对成牙骨质细胞生物学活性的影响,牙骨质合成中的主要效应细胞。
方法：用不同浓度的人牙周膜干细胞来源的外泌体培养OCCM-30成牙骨质细胞。OCCM-30细胞增殖,迁移,并检查了成水泥矿化，以及与成骨细胞矿化相关因子的基因和蛋白质表达。
结果：外泌体促进了迁移，扩散,和OCCM-30细胞的矿化。外泌体处理组显着增加了骨水泥相关基因和蛋白质的表达。此外，外泌体给药可增强p-PI3K和p-AKT的表达。用PI3K/AKT抑制剂治疗可显着减弱成骨细胞因子的基因和蛋白质表达，这种作用被外泌体给药部分逆转。
结论：人牙周膜干细胞来源的外泌体可通过PI3K/AKT信号通路促进成牙骨质细胞的活性，为促进正畸致炎性牙根吸收的修复过程提供科学依据。
方法：用不同浓度的人牙周膜干细胞来源的外泌体培养OCCM-30成牙骨质细胞。OCCM-30细胞增殖,迁移,并检查了成水泥矿化，以及与成骨细胞矿化相关因子的基因和蛋白质表达。
结果：外泌体促进了迁移，扩散,和OCCM-30细胞的矿化。外泌体处理组显着增加了骨水泥相关基因和蛋白质的表达。此外，外泌体给药可增强p-PI3K和p-AKT的表达。用PI3K/AKT抑制剂治疗可显着减弱成骨细胞因子的基因和蛋白质表达，这种作用被外泌体给药部分逆转。
结论：人牙周膜干细胞来源的外泌体可通过PI3K/AKT信号通路促进成牙骨质细胞的活性，为促进正畸致炎性牙根吸收的修复过程提供科学依据。
