PDF(Pubmed)
Abstract：
MADS-box transcription regulators play important roles in plant growth and development. However, very few MADS-box genes have been isolated in the genus Taraxacum, which consists of more than 3000 species. To explore their functions in the promising natural rubber (NR)-producing plant Taraxacum kok-saghyz (TKS), MADS-box genes were identified in the genome of TKS and the related species Taraxacum mongolicum (TM; non-NR-producing) via genome-wide screening. In total, 66 TkMADSs and 59 TmMADSs were identified in the TKS and TM genomes, respectively. From diploid TKS to triploid TM, the total number of MADS-box genes did not increase, but expansion occurred in specific subfamilies. Between the two genomes, a total of 11 duplications, which promoted the expansion of MADS-box genes, were identified in the two species. TkMADS and TmMADS were highly conserved, and showed good collinearity. Furthermore, most TkMADS genes exhibiting tissue-specific expression patterns, especially genes associated with the ABCDE model, were preferentially expressed in the flowers, suggesting their conserved and dominant functions in flower development in TKS. Moreover, by comparing the transcriptomes of different TKS lines, we identified 25 TkMADSs related to biomass formation and 4 TkMADSs related to NR content, which represented new targets for improving the NR yield of TKS.
摘要：
MADS-box转录调控因子在植物生长发育中发挥重要作用。然而,在蒲公英属中分离出的MADS-box基因很少，由3000多个物种组成。为了探索它们在有前途的天然橡胶（NR）生产植物蒲公英（TKS）中的功能，通过全基因组筛选，在TKS和相关物种蒲公英（TM；不产生NR）的基因组中鉴定了MADS-box基因。总的来说,在TKS和TM基因组中鉴定出66个TkMADSs和59个TmMADSs，分别。从二倍体TKS到三倍体TM，MADS-box基因的总数没有增加，但扩张发生在特定的亚家族。在两个基因组之间，共11次重复,这促进了MADS-box基因的扩增，在这两个物种中被发现。TkMADS和TmMADS高度保守，表现出良好的共线性。此外，大多数TkMADS基因表现出组织特异性表达模式，特别是与ABCDE模型相关的基因，优先在花朵中表达，表明它们在TKS花卉发育中的保守和主导作用。此外，通过比较不同TKS系的转录组，我们确定了25个与生物量形成相关的TkMADSs和4个与NR含量相关的TkMADSs，这代表了提高TKSNR产率的新目标。
