PDF(Pubmed)
Abstract：
Cashmere, a keratinised product of secondary hair follicles (SHFs) in cashmere goats, holds an important place in international high-end textiles. However, research on the complex molecular and signal regulation during the development and growth of hair follicles (HFs), which is essential for the development of the cashmere industry, is limited. Moreover, increasing evidence indicates that non-coding RNAs (ncRNAs) participate in HF development. Herein, we systematically investigated a competing endogenous RNA (ceRNA) regulatory network mediated by circular RNAs (circRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) in skin samples of cashmere goat embryos, using whole-transcriptome sequencing technology. We obtained 6468, 394, and 239 significantly differentially expressed mRNAs, circRNAs, and miRNAs, respectively. These identified RNAs were further used to construct a ceRNA regulatory network, mediated by circRNAs, for cashmere goats at a late stage of HF development. Among the molecular species identified, miR-184 and fibroblast growth factor (FGF) 10 exhibited competitive targeted interactions. In secondary HF dermal papilla cells (SHF-DPCs), miR-184 promotes proliferation, inhibits apoptosis, and alters the cell cycle via the competitive release of FGF10. This study reports that FGF10 and its interaction with ncRNAs significantly affect SHF-DPCs, providing a reference for research on the biology of HFs in cashmere goats and other mammals.
摘要：
羊绒,绒山羊次级毛囊（SHF）的角化产物，在国际高端纺织品中占有重要地位。然而,毛囊（HFs）发育和生长过程中复杂的分子和信号调控研究，这对羊绒行业的发展至关重要，是有限的。此外，越来越多的证据表明，非编码RNA（ncRNAs）参与HF的发展。在这里，我们系统地研究了由环状RNA（circRNAs）介导的竞争性内源性RNA（ceRNA）调控网络，microRNAs(miRNAs),和羊绒山羊胚胎皮肤样本中的信使RNA（mRNA），使用全转录组测序技术。我们获得了6468、394和239个显著差异表达的mRNA，circRNAs,和miRNAs，分别。这些鉴定的RNA进一步用于构建ceRNA调控网络，由circRNAs介导，用于HF发展后期的绒山羊。在确定的分子种类中，miR-184和成纤维细胞生长因子(FGF)10表现出竞争性靶向相互作用。在继发性HF真皮乳头细胞（SHF-DPCs）中，miR-184促进增殖，抑制细胞凋亡，并通过FGF10的竞争性释放改变细胞周期。本研究报告FGF10及其与ncRNAs的相互作用显著影响SHF-DPCs,为绒山羊和其他哺乳动物的HFs生物学研究提供参考。
