PDF(Pubmed)
Abstract：
The cultivation of marine invertebrate cells in vitro has garnered significant attention due to the availability of diverse cell types and cellular potentialities in comparison to vertebrates and particularly in response to the demand for a multitude of applications. While cells in the colonial urochordate Botryllus schlosseri have a very high potential for omnipotent differentiation, no proliferating cell line has been established in Botryllus, with results indicating that cell divisions cease 24-72 h post initiation. This research assessed how various Botryllus blood cell types respond to in vitro conditions by utilizing five different refinements of cell culture media (TGM1-TGM5). During the initial week of culture, there was a noticeable medium-dependent increase in the proliferation and viability of distinct blood cell types. Within less than one month from initiation, we developed medium-specific primary cultures, a discovery that supports larger efforts to develop cell type-specific cultures. Specific cell types were easily distinguished and classified based on their natural fluorescence properties using confocal microscopy. These results are in agreement with recent advances in marine invertebrate cell cultures, demonstrating the significance of optimized nutrient media for cell culture development and for cell selection.
摘要：
与脊椎动物相比,特别是响应于多种应用的需求,由于多种细胞类型和细胞潜力的可用性,在体外培养海洋无脊椎动物细胞已经引起了极大的关注。虽然殖民地尿索定酸Botryllusschlosseri中的细胞具有非常高的全能分化潜力，Botryllus尚未建立增殖细胞系，结果表明，细胞分裂在开始后24-72小时停止。这项研究通过利用五种不同的细胞培养基（TGM1-TGM5）来评估各种Botryllus血细胞类型对体外条件的反应。在文化的最初一周，不同类型血细胞的增殖和生存力显著增加.在入会后不到一个月内，我们开发了特定于培养基的原代培养物，这一发现支持开发细胞类型特异性培养物的更大努力。使用共聚焦显微镜根据其天然荧光特性容易区分和分类特定细胞类型。这些结果与海洋无脊椎动物细胞培养的最新进展一致，证明了优化的营养培养基对细胞培养开发和细胞选择的重要性。
