PDF(Pubmed)
Abstract：
Postnatal bone fractures of the growth plate (GP) are often associated with regenerative complications such as growth impairment. In order to understand the underlying processes of trauma-associated growth impairment within postnatal bone, an ex vivo rat femur slice model was developed. To achieve this, a 2 mm horizontal cut was made through the GP of rat femur prior to the organotypic culture being cultivated for 15 days in vitro. Histological analysis showed disrupted endochondral ossification, including disordered architecture, increased chondrocyte metabolic activity, and a loss of hypertrophic zone throughout the distal femur. Furthermore, altered expression patterns of Col2α1, Acan, and ColX, and increased chondrocyte metabolic activity in the TZ and MZ at day 7 and day 15 postinjury were observed. STEM revealed the presence of stem cells, fibroblasts, and chondrocytes within the injury site at day 7. In summary, the findings of this study suggest that the ex vivo organotypic GP injury model could be a valuable tool for investigating the underlying mechanisms of GP regeneration post-trauma, as well as other tissue engineering and disease studies.
摘要：
生长板（GP）的出生后骨折通常与再生并发症有关，例如生长障碍。为了了解出生后骨骼中与创伤相关的生长障碍的潜在过程，建立了离体大鼠股骨切片模型。为了实现这一点，在器官型培养物体外培养15天之前，通过大鼠股骨的GP进行2mm水平切割。组织学分析显示软骨内骨化破坏,包括无序的建筑，软骨细胞代谢活性增加，以及整个股骨远端肥厚区的丧失。此外，Col2α1、Acan、还有Colx,在损伤后第7天和第15天观察到TZ和MZ中软骨细胞代谢活性增加。STEM揭示了干细胞的存在，成纤维细胞,和在第7天损伤部位内的软骨细胞。总之,这项研究的结果表明，离体器官型GP损伤模型可能是研究创伤后GP再生的潜在机制的有价值的工具，以及其他组织工程和疾病研究。
