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Abstract：
The odontogenic differentiation of dental pulp stem cells (DPSCs), which is vital for tooth regeneration, was regulated by various functional molecules. In recent years, a growing body of research has shown that miRNAs play a crucial role in the odontogenic differentiation of human dental pulp stem cells (hDPSCs). However, the mechanisms by which miRNAs regulated odontogenic differentiation of hDPSCs remained unclear, and the application of miRNAs in reparative dentin formation in vivo was also rare. In this study, we first discovered that miR-3074-3p had an inhibitory effect on odontogenic differentiation of hDPSCs and antagomiR-3074-3p-conjugated PEI-AuNPs effectively promoted odontogenic differentiation of hDPSCs in vitro. AntagomiR-3074-3p-conjugated PEI-AuNPs was further applied to the rat pulp-capping model and showed the increased formation of restorative dentin. In addition, the results of lentivirus transfection in vitro suggested that FKBP9 acted as the key target of miR-3074-3p in regulating the odontogenic differentiation of hDPSCs. These findings might provide a new strategy and candidate target for dentin restoration and tooth regeneration.
摘要：
牙髓干细胞（DPSCs）的牙源性分化，这对牙齿再生至关重要，受到各种功能分子的调控。近年来,越来越多的研究表明，miRNA在人类牙髓干细胞（hDPSC）的牙源性分化中起着至关重要的作用。然而,miRNA调控hDPSC牙源性分化的机制尚不清楚,miRNAs在修复性牙本质形成中的应用也很少。在这项研究中,我们首次发现miR-3074-3p对hDPSCs牙源性分化有抑制作用,而antagomiR-3074-3p偶联的PEI-AuNPs在体外有效促进hDPSCs牙源性分化.将AntagomiR-3074-3p缀合的PEI-AuNP进一步应用于大鼠盖髓模型，并显示修复性牙本质的形成增加。此外,慢病毒体外转染结果表明，FKBP9作为miR-3074-3p调控hDPSCs牙源性分化的关键靶点。这些发现可能为牙本质修复和牙齿再生提供新的策略和候选目标。
