PDF(Pubmed)
Abstract：
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a global threat since 2020. The emergence of the Omicron variant in 2021, which replaced Delta as the dominant variant of concern, has had a significant adverse impact on the global economy and public health. During this period, Zhejiang Province implemented dynamic zeroing and focused on preventing imported cases. This study aimed to gain clear insight into the characteristics of imported COVID-19 cases in Zhejiang Province.
We conducted a systematic molecular epidemiological analysis of 146 imported cases between July 2021 and November 2022 in Zhejiang Province. Virus samples with cycle threshold (Ct) value less than 32 were performed next generation sequencing. Basing the whole genome sequence obtained after quality control and assembly of reads, the whole genome variation map and phylogenetic tree were constructed and further analyzed.
Our study identified critical months and populations for surveillance, profiled the variation of various lineages, determined the evolutionary relationships among various lineages of SARS-CoV-2, and compared the results in Zhejiang with those obtained worldwide during this period.
The continuous molecular epidemiological surveillance of imported cases of COVID-19 in Zhejiang Province during 2021 to 2022 is consistent with the global epidemic trend.
We conducted a systematic molecular epidemiological analysis of 146 imported cases between July 2021 and November 2022 in Zhejiang Province. Virus samples with cycle threshold (Ct) value less than 32 were performed next generation sequencing. Basing the whole genome sequence obtained after quality control and assembly of reads, the whole genome variation map and phylogenetic tree were constructed and further analyzed.
Our study identified critical months and populations for surveillance, profiled the variation of various lineages, determined the evolutionary relationships among various lineages of SARS-CoV-2, and compared the results in Zhejiang with those obtained worldwide during this period.
The continuous molecular epidemiological surveillance of imported cases of COVID-19 in Zhejiang Province during 2021 to 2022 is consistent with the global epidemic trend.
摘要：
■自2020年以来，由严重急性呼吸道综合症冠状病毒2（SARS-CoV-2）引起的2019年冠状病毒病（COVID-19）大流行一直是全球威胁。2021年Omicron变体的出现，取代Delta成为主要的关注变体，对全球经济和公共卫生产生了重大不利影响。在此期间,浙江省实施动态归零，重点防范输入病例。本研究旨在深入了解浙江省输入性COVID-19病例的特点。
■我们对浙江省2021年7月至2022年11月的146例输入性病例进行了系统的分子流行病学分析。循环阈值(Ct)值小于32的病毒样品进行下一代测序。基于质量控制和读段组装后获得的全基因组序列，构建了全基因组变异图谱和系统发育树,并进行了进一步分析。
■我们的研究确定了监测的关键月份和人群，描绘了各种血统的变化，确定了SARS-CoV-2各个谱系之间的进化关系，并将浙江的结果与在此期间的全球结果进行了比较。
■浙江省2021-2022年COVID-19输入病例的连续分子流行病学监测与全球流行趋势一致。
■我们对浙江省2021年7月至2022年11月的146例输入性病例进行了系统的分子流行病学分析。循环阈值(Ct)值小于32的病毒样品进行下一代测序。基于质量控制和读段组装后获得的全基因组序列，构建了全基因组变异图谱和系统发育树,并进行了进一步分析。
■我们的研究确定了监测的关键月份和人群，描绘了各种血统的变化，确定了SARS-CoV-2各个谱系之间的进化关系，并将浙江的结果与在此期间的全球结果进行了比较。
■浙江省2021-2022年COVID-19输入病例的连续分子流行病学监测与全球流行趋势一致。
